| 產品名稱 |
D1B |
| 商品貨號 |
B164315 |
| Organism |
Mus musculus, mouse |
| Tissue |
spleen |
| Cell Type |
erythroblast |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease |
leukemia |
| Strain |
DBA/2 |
| Applications |
This line was derived from a leukemia induced by B-tropic Friend murine leukemia virus. The cells are negative for H-2d (H-2d-), surface immunoglobulin (sIg-), Ia8 (Ia8-) and Thy-1.2 (Thy-1.2-). Tested and found negative for ectromelia virus (mousepox). |
| Storage Conditions |
liquid nitrogen vapor phase |
| Derivation |
This line was derived from a leukemia induced by B-tropic Friend murine leukemia virus. |
| Genes Expressed |
globin mRNA; hemoglobin |
| Cellular Products |
globin mRNA; hemoglobin |
| Tumorigenic |
Yes |
| Effects |
Yes, in DBA/2 mice |
| Comments |
This line was derived from a leukemia induced by B-tropic Friend murine leukemia virus. The cells are negative for H-2d (H-2d-), surface immunoglobulin (sIg-), Ia8 (Ia8-) and Thy-1.2 (Thy-1.2-). Erythroid differentiation and hemoglobin synthesis are inducible by DMSO. Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium |
RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate and supplemented with 0.01 mM 2-mercaptoethanol, 90%; fetal bovine serum, 10%
|
| Subculturing |
Protocol: Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10(5) cells/ml and maintain between 1 X 10(5) and 1 X 10 exp6 cells/ml. The cells may also be grown as ascites in syngeneic mice.Inject 2 X 10(5) cell per mouse and harvest as soon as abdominal enlargement is observed (about 10 days). Medium Renewal: Every 2 to 3 days |
| Cryopreservation |
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions |
Temperature: 37.0°C |
| Name of Depositor |
B Chesebro |
| Deposited As |
Mus musculus |
| References |
Chesebro B, et al. Lack of erythroid characteristics in Ia-positive leukemia cell lines induced by Friend murine leukemia virus: brief communication. J. Natl. Cancer Inst. 60: 239-242, 1978. PubMed: 564410
Chesebro B, et al. Characterization of Ia8 antigen, thy-1.2 antigen, complement receptors, and virus production in a group of murine virus-induced leukemia cell lines. J. Immunol. 117: 1267-1274, 1976. PubMed: 185295
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