| Comments |
This line was derived from a leukemia induced by N-tropic Friend murine leukemia virus. The cells are negative for surface immunoglobulin (sIg-) and Thy-1.2 (Thy-1.2-). Erythroid differentiation and hemoglobin synthesis are NOT inducible by DMSO. Tested and found negative for ectromelia virus (mousepox). |
| Subculturing |
Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. The cells may also be grown as ascites in syngeneic mice. Inject 2 X 10 exp5 cell per mouse and harvest as soon as abdominal enlargement is observed (about 10 days). |
| References |
Chesebro B, et al. Lack of erythroid characteristics in Ia-positive leukemia cell lines induced by Friend murine leukemia virus: brief communication. J. Natl. Cancer Inst. 60: 239-242, 1978. PubMed: 564410
Chesebro B, et al. Characterization of Ia8 antigen, thy-1.2 antigen, complement receptors, and virus production in a group of murine virus-induced leukemia cell lines. J. Immunol. 117: 1267-1274, 1976. PubMed: 185295
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