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Daudi

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編號:B164333

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產(chǎn)品名稱	Daudi
商品貨號	B164333
Organism	Homo sapiens, human
Tissue	peripheral blood
Cell Type	B lymphoblast
Product Format	frozen
Morphology	lymphoblast
Culture Properties	suspension
Biosafety Level	2 [Cells contain Herpesvirus] Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease	Burkitt's lymphoma
Age	16 years
Gender	male
Ethnicity	Black
Applications	Daudi is a well characterized B lymphoblast cell line which has been employed extensively in studies of mechanisms of leukemogenesis. The cell line is also a suitable transfection host
Storage Conditions	liquid nitrogen vapor phase
Karyotype	Male human karyotype with stemline number of 46. The karyotype is diploid in 66% of the cells and is stable within the stemline. Note: Cytogenetic information is based on initial seed stock at ATCC. Cytogenetic instability has been reported in the literature for some cell lines.
Derivation	The Daudi line was derived from a 16-year-old Black male with Burkitt's lymphoma by E. Klein and G. Klein in May, 1967.
Clinical Data	16 years Black male
Receptor Expression	complement, expressed Fc, expressed
Tumorigenic	Yes, in nude mice; forms colonies in agarose
Effects	Yes, in agarose Yes, in nude mice
Comments	The cells are negative for beta-2-microglobulin. They are positive for EBNA, VCA and Surface immunoglobulin (sIg+). ATCC confirmed this cell line is positive for the presence of EBV viral DNA sequences via PCR.
Complete Growth Medium	The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
Subculturing	Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 3 to 5 x 10⁵ viable cells/mL. Corning^® T-75 flasks (catalog #431464) are recommended for subculturing this product. Interval: Maintain cell density between 3 x 10⁵ and 2 to 3 x 10⁶ viable cells/mL. Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Cryopreservation	Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase
Culture Conditions	Temperature: 37°C Atmosphere: air, 95%; carbon dioxide (CO₂), 5%
STR Profile	Amelogenin: X,Y CSF1PO: 12 D13S317: 11,12 D16S539: 10,12 D5S818: 8,13 D7S820: 8,10 THO1: 6,7 TPOX: 8,11 vWA: 15,17
Isoenzymes	G6PD, B
Isotype	IgM
Name of Depositor	G Klein
Deposited As	Homo sapiens
Year of Origin	May, 1967
References	Ohsugi Y, et al. Tumorigenicity of human malignant lymphoblasts: comparative study with unmanipulated nude mice, antilymphocyte serum-treated nude mice, and X- irradiated nude mice. J. Natl. Cancer Inst. 65: 715-718, 1980. PubMed: 6932523 Klein E, et al. Surface IgM-kappa specificity on a Burkitt lymphoma cell in vivo and in derived culture lines. Cancer Res. 28: 1300-1310, 1968. PubMed: 4174339 Huber C, et al. Surface receptors on human haematopoietic cell lines. Clin. Exp. Immunol. 25: 367-378, 1976. PubMed: 963908 Nilsson K, et al. Tumorigenicity of human hematopoietic cell lines in athymic nude mice. Int. J. Cancer 19: 337-344, 1977. PubMed: 14896 Gao Y, et al. Induction of an exceptionally high-level, nontranslated, Epstein-Barr virus-encoded polyadenylated transcript in the uurkitts lymphoma line Daudi. J. Virol. 71: 84-94, 1997. PubMed: 8985326 Cuthbert JA, Lipsky PE. Regulation of proliferation and Ras localization in transformed cells by products of mevalonate metabolism. Cancer Res. 57: 3498-3504, 1997. PubMed: 9270019 Yamaguchi Y, et al. Biochemical characterization and intracellular localization of the Menkes disease protein. Proc. Natl. Acad. Sci. USA 93: 14030-14035, 1996. PubMed: 8943055 Lewis JA, et al. Inhibition of mitochondrial function by interferon. J. Biol. Chem. 271: 13184-13190, 1996. PubMed: 8662694 Montoya JG, et al. Human CD4+ and CD8+ T lymphocytes are both cytotoxic to Toxoplasma gondii-infected cells. Infect. Immun. 64: 176-181, 1996. PubMed: 8557337
Cross References	Nucleotide (GenBank) : U07990 Human Burkitt's lymphoma immunoglobulin kappa light chain mRNA, partial cds. Nucleotide (GenBank) : U07987 Human Burkitt's lymphoma immunoglobulin IgM heavy chain Fd fragment mRNA, partial cds.

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