| 產(chǎn)品名稱 |
Dede |
| 商品貨號(hào) |
B164344 |
| Organism |
Cricetulus griseus, hamster, Chinese |
| Tissue |
lung |
| Product Format |
frozen |
| Morphology |
fibroblast |
| Culture Properties |
adherent |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Age |
adult |
| Gender |
female |
| Clinical Data |
female |
| Virus Resistance |
poliovirus 1 |
| Complete Growth Medium |
McCoy?s 5a medium (modified) with 1.5 mM L-glutamine adjusted to contain 2.2 g/L sodium bicarbonate, 80%; fetal bovine serum, 20%
|
| Subculturing |
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:10 is recommended
Medium Renewal: Twice per week
Remove medium, add fresh 0.25% trypsin solution for 3 to 5 minutes, remove trypsin and allow culture to sit at room temperature for 10 to 20 minutes. Add fresh medium, aspirate and dispense into new flasks.
Note: If necessary, the serum can be reduced to 10%. |
| Culture Conditions |
Atmosphere: air, 95%; carbon dioxide (CO2), 5% |
| Name of Depositor |
TC Hsu |
| Deposited As |
Cricetulus griseus |
| References |
Stich HF, et al. Viruses and mammalian chromosomes. I. Localization of chromosome aberrations after infection with Herpes simplex virus. Virology 22: 439-445, 1964. PubMed: 14171598
Rapp F, Hsu TC. Viruses and mammalian chromosomes. IV. Replication of Herpes simplex virus in diploid Chinese hamster cells. Virology 25: 401-411, 1965. PubMed: 14328610
Stich HF, et al. Viruses and mammalian chromosomes; chromosome aberrations by human adenovirus type 12. Exp. Cell Res. 34: 400-403, 1964. PubMed: 14165598
|
| Cross References |
Nucleotide (GenBank) :
X86458
C.griseus mRNA for serotonin receptor 1B.
Nucleotide (GenBank) :
X61958
C.longicaudatus mRNA for thrombin receptor.
Nucleotide (GenBank) :
X53791
Chinese hamster mRNA for 5-HT type 2 serotonin receptor.
|
