| 產品名稱 |
DI TNC1 |
| 商品貨號 |
B164358 |
| Organism |
Rattus norvegicus, rat |
| Tissue |
brain, diencephalon |
| Cell Type |
astrocyte, type 1 phenotype |
| Product Format |
frozen |
| Morphology |
fibroblast |
| Culture Properties |
adherent |
| Biosafety Level |
2 [Cells contain polyomavirus DNA sequences]
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease |
normal |
| Age |
neonate |
| Strain |
Sprague-Dawley |
| Derivation |
The DI TNC1 cell line was established from primary cultures of type 1 astrocytes from brain diencephalon tissue of 1 day old rats.
The cultures were transfected 3 days after initial plating with a DNA construct containing the oncogenic early region of SV40 under the transcriptional control of the human GFAP promoter (pGFA-SV-Tt).
|
| Genes Expressed |
alpha 2 macroglobulin; transferrin |
| Cellular Products |
alpha 2 macroglobulin; transferrin |
| Tumorigenic |
No |
| Effects |
No, The cells were not tumorigenic in immunosuppressed mice, but did form colonies in semisolid medium. |
| Comments |
The cells retain characteristics consistent with the phenotype of type 1 astrocytes including glial fibrillary acidic protein (GFAP) immunoreactivity, and a high affinity uptake mechanism for gamma aminobutyric acid (GABA) that is inhibitable by beta alanine.?
The cells produce alpha 2 macroglobulin in amounts similar to those found in primary astrocytes but produce transferrin in much lesser amounts.?
This line does not produce proenkephalin A, does not express the O4 or A2B5 epitopes characteristic of type 2 astrocytes, and does not express galactocerebroside.
SV40 T antigen was found in the nuclei of over 95% of the cells examined by immunostaining.
|
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing |
Remove spent medium, add fresh 0.25% trypsin, 0.03% EDTA solution, rinse and remove.
Add fresh trypsin solution, (1 to 2 mL) and allow the cells to sit at room temperature (or at 37°C) until the cells detach. Add fresh medium, aspirate and dispense into new flasks.
NOTE: The cells will come off in sheets and are difficult to dissociate.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: Every 2 to 3 days
|
| Cryopreservation |
culture medium 95%; DMSO, 5% |
| Culture Conditions |
Temperature: 37°C |
| Name of Depositor |
CF Deschepper |
| Deposited As |
Rattus sp. |
| References |
Radany EH, et al. Directed establishment of rat brain cell lines with the phenotypic characteristics of type 1 astrocytes. Proc. Natl. Acad. Sci. USA 89: 6467-6471, 1992. PubMed: 1378628
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