| 產品名稱 |
DREG200 |
| 商品貨號 |
B164367 |
| Organism |
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications |
It cross-reacts with rabbit L-selectin. |
| Derivation |
Spleen cells were fused with Sp2/0-Ag14 myeloma cells. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against human L-selectin (CD62L, LECAM, LAM-1, Leu8) |
| Cellular Products |
immunoglobulin; monoclonal antibody; against human L-selectin (CD62L, LECAM, LAM-1, Leu8) |
| Comments |
Animals were immunized with supernatant from phorbol myristic acid activated human peripheral blood leukocytes. Spleen cells were fused with Sp2/0-Ag14 myeloma cells. The antibody partially blocks lymphocyte adhesion to high enothelial venules of peripheral lymph nodes. It cross-reacts with rabbit L-selectin. |
| Complete Growth Medium |
Culture Medium: RPMI 1640 medium with 4.5 g/L glucose, 10 mM HEPES, 2 mM
L-glutamine, 1 mM sodium pyruvate and 0.1 mM non-essential amino acids
supplemented with 0.05 mM 2-mercaptoethanol, 90%; fetal bovine serum, 10%. |
| Subculturing |
Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Cryopreservation |
Culture medium, 95%; DMSO, 5% |
| Isotype |
IgG1 |
| Name of Depositor |
EC Butcher |
| Deposited As |
mouse (B cell); mouse (myeloma) |
| References |
Kishimoto TK, et al. Identification of a human peripheral lymph node homing receptor: a rapidly down-regulated adhesion molecule. Proc. Natl. Acad. Sci. USA 87: 2244-2248, 1990. PubMed: 2179952
von Andrian UH, et al. Two-step model of leukocyte-endothelial cell interaction in inflammation: distinct roles for LECAM-1 and the leukocyte beta 2 integrins in vivo. Proc. Natl. Acad. Sci. USA 88: 7538-7542, 1991. PubMed: 1715568
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