| 產(chǎn)品名稱 | EGC/PK060399egfr |
|---|---|
| 商品貨號 | B164395 |
| Organism | Rattus norvegicus, rat |
| Tissue | jejunum |
| Cell Type | enteroglial |
| Product Format | frozen |
| Morphology | glial |
| Culture Properties | adherent |
| Biosafety Level | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Age | adult adult |
| Gender | male |
| Strain | Sprague-Dawley |
| Storage Conditions | liquid nitrogen vapor phase |
| Clinical Data | male |
| Genes Expressed | glial fibrillary acidic protein (GFAP); S-100 protein and vimentin |
| Cellular Products | glial fibrillary acidic protein (GFAP); S-100 protein and vimentin Ref   Ruhl A, et al. Isolation of enteric glia and establishment of transformed enteroglial cell lines from the myenteric plexus of adult rat. Neurogastroenterol. Motil. 13: 95-106, 2001. PubMed: 11169131 |
| Comments | The cells display robust glial fibrillary acidic protein (GFAP), S-100 and vimentin immunoreactivities, but no Thy-1.1, desmin, smooth muscle alpha-actin or C3 complement receptor immunoreactivity. |
| Complete Growth Medium | The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing | Protocol: Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks. Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:6 is recommended Medium Renewal: Every 2 to 3 days |
| Cryopreservation | Freeze medium: Complete growth medium 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions | Temperature: 37.0°C |
| Name of Depositor | A Ruhl |
| Deposited As | rat |
| Year of Origin | March 6, 1999 |
| References | Ruhl A, et al. Isolation of enteric glia and establishment of transformed enteroglial cell lines from the myenteric plexus of adult rat. Neurogastroenterol. Motil. 13: 95-106, 2001. PubMed: 11169131 The EGC/PK060399egfr enteroglial cell line was established on March 6, 1999 from adult rat myenteric plexus from the jejunum. Purified primaries were transfected with supernants from the virus producing cell line GPE-egfr-neu. GPE-egfr-neu cells contain the chimeric gene egfr/neu and the neomycin-resistance gene neo under the control of the long-terminal repeats of the mouse Moloney leukaemia virus. The egfr/neu gene codes for the extracellular domain of the human EGF receptor and the intracellular tyrosine kinase domain of the proto-oncogene c-neu. |
| 梅經(jīng)理 | 17280875617 | 1438578920 |
| 胡經(jīng)理 | 13345964880 | 2438244627 |
| 周經(jīng)理 | 17757487661 | 1296385441 |
| 于經(jīng)理 | 18067160830 | 2088210172 |
| 沈經(jīng)理 | 19548299266 | 2662369050 |
| 李經(jīng)理 | 13626845108 | 972239479 |
