| 產(chǎn)品名稱 |
FB2 |
| 商品貨號(hào) |
B164438 |
| Organism |
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications |
The antibody reacts with several phosphorylated proteins on Western blots and in immunoprecipitation reactions. |
| Derivation |
Spleen cells were fused with FOX-NY myeloma cells. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against phosphotyrosine |
| Cellular Products |
immunoglobulin; monoclonal antibody; against phosphotyrosine |
| Comments |
Spleen cells were fused with FOX-NY myeloma cells. The antibody reacts with several phosphorylated proteins on Western blots and in immunoprecipitation reactions. Thymidine and aminopterin are added to the culture medium to stabilize the presence of the immunoglobulin heavy chain locus. |
| Complete Growth Medium |
RPMI 1640 medium with 10 mM HEPES, 0.0039 mg/ml thymidine, 176 ng/ml aminopterin and 0.075 mM adenine, 90%; fetal bovine serum, 10%
|
| Subculturing |
Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Culture Conditions |
Temperature: 37.0°C |
| Isotype |
IgG3; kappa light chain |
| Name of Depositor |
J Burkhart, LT Williams |
| Deposited As |
mouse (B cell); mouse (myeloma) |
| References |
Frackelton AR Jr., et al. Evidence for the platelet-derived growth factor-stimulated tyrosine phosphorylation of the platelet-derived growth factor receptor in vivo. Immunopurification using a monoclonal antibody to phosphotyrosine. J. Biol. Chem. 259: 7909-7915, 1984. PubMed: 6203898
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