| 產(chǎn)品名稱 |
FeT-J |
| 商品貨號(hào) |
B164453 |
| Organism |
Felis catus, cat |
| Tissue |
peripheral blood mononuclear cells (PBMC) |
| Cell Type |
T lymphocyte |
| Product Format |
frozen |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disclosure |
This material is cited in a US or other Patent and may not be used to
infringe the claims. Depending on the wishes of the Depositor, ATCC may be
required to inform the Patent Depositor of the party to which the material was
furnished. This material may not have been produced or characterized by ATCC. |
| Derivation |
The cell line was derived from a specific pathogen free (SPF) cat [U.S. Pat. 5,846,825]. |
| Comments |
This feline T-cell line is susceptible to infection by feline immunodeficiency virus (FIV) [U.S. Pat. 5,846,825]. The FeT-1C cell line (ATCC CRL-11968) is IL-2 dependent, whereas the FeT-J cell line (ATCC CRL-11967) is IL-2 independent. |
| Complete Growth Medium |
RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate and supplemented with 0.05 mM 2-mercaptoethanol and 10% heat-inactivated fetal bovine serum
|
| Subculturing |
Cultures can be maintained by addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension in fresh medium at 5 x 105 viable cells/mL. Maintain cultures at cell concentrations between 5 X 105 and 2 X 106 viable cells/mL.
Medium Renewal: Every 3 to 4 days |
| Cryopreservation |
culture medium 95%; DMSO, 5% |
| Culture Conditions |
Temperature: 37°C |
| Name of Depositor |
University of Florida, Gainesville |
| Deposited As |
cat |
| U.S. Patent Number |
|
| References |
Yamamoto JK. Feline derived T-cell lines for producing FIV. US Patent 5,846,825 dated Dec 8 1998
Hohdatsu T, et al. Comparative study of the cell tropism of feline immunodeficiency virus isolates of subtypes A, B and D classified on the basis of the env gene V3-V5 sequence. J. Gen. Virol. 77: 93-100, 1996. PubMed: 8558132
|
