| 產(chǎn)品名稱 |
FHs 173We |
| 商品貨號(hào) |
B164458 |
| Organism |
Homo sapiens, human |
| Tissue |
fetus, whole |
| Product Format |
frozen |
| Morphology |
fibroblast |
| Culture Properties |
adherent |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease |
normal |
| Age |
fetus |
| Gender |
male |
| Ethnicity |
Black |
| Storage Conditions |
liquid nitrogen vapor phase |
| Clinical Data |
male Black fetus |
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing |
Protocol: Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks. Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended Medium Renewal: 2 to 3 times per week |
| Cryopreservation |
Freeze medium: Complete growth medium, 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions |
Temperature: 37.0°C |
| STR Profile |
Amelogenin: X,Y CSF1PO: 10,11 D13S317: 12 D16S539: 9,12 D5S818: 10,12 D7S820: 8,11 THO1: 7,8 TPOX: 8,9 vWA: 15,16 |
| Isoenzymes |
AK-1, 1 ES-D, 1-2 G6PD, B GLO-I, 2 PGM1, 1 PGM3, 1-2 |
| Name of Depositor |
RB Owens |
| Deposited As |
Homo sapiens |
