Strain: Wistar (B cell); BALB/c (myeloma) Tissue: B lymphocyte; hybridoma Morphology: lymphoblast Depositors: E.C. Butcher; D. Andrew Products: immunoglobulin; monoclonal antibody; against murine b7 integrin FreezeMedium: Culture medium, 95%; DMSO, 5% FluidRenewal: Every 2 to 3 days SubCulturing: Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/mL and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/mL. Growth Properties: suspension Comments: Animals were immunized with cells of the TK1 murine T cell lymphoma. Spleen cells were fused with Sp2/0-Ag14 myeloma cells. The antibody reacts with human and mouse b7 integrin. It partially blocks lymphocyte binding to MAdCAM-1, and a4b7 integrin binding to VCAM-1 and fibronectin. It is useful for both immunofluorescence and immunohistological staining. NOTE: a feeder layer of mouse peritoneal macrophages or X-irradiated mouse fibroblasts (see ATCC 56-X, irradiated STO cells) must be used in order to successfully recover these cells from the frozen state. References: ????????? RF33063: Berlin C et al. Alpha 4 beta 7 integrin mediates lymphocyte binding to the mucosal vascular addressin MAdCAM-1. Cell 74: 185-195, 1993 PubMed: 93327426 ????????? RF33868: Andrew DP et al. Distinct but overlapping epitopes are involved in a4b7 - mediated adhesion to vascular cell adhesion molecule-1, mucosal addressin-1, fibronectin, and lymphocyte aggregation. J. Immunol. 153: 3847-3861, 1994 PubMed: 95015875 |
