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H2.8

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編號(hào):B164528

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產(chǎn)品名稱	H2.8
商品貨號(hào)	B164528
Organism	Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Tissue	spleen
Cell Type	hybridoma:lymphoblast B lymphocyte
Product Format	frozen
Morphology	lymphoblast
Culture Properties	suspension
Biosafety Level	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Age	adult
Strain	BALB/c
Applications	ELISA detection zona pellucida glycoprotein 2 ZP2, zona pellucida protein A Western Blot zona pellucida glycoprotein 2 ZP2, zona pellucida protein A detection of zona pellucida glycoprotein 2 ZP2, zona pellucida protein A
Storage Conditions	liquid nitrogen vapor phase
Genes Expressed	zona pellucida glycoprotein 2
Complete Growth Medium	Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose supplemented with 10 mM HEPES, 0.15 mg/ml oxaloacetate, 0.05 mg/ml pyruvate, 0.0082 mg/ml bovine insulin and 0.05 mM 2-mercaptoethanol, 80%; fetal bovine serum, 20%
Subculturing	Cultures can be maintained by addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation of the suspension with subsequent resuspension in fresh medium at 2 x 10⁵ viable cells/mL. Add medium as the cell density increases. Maintain cultures at cell concentrations between 1 x 10⁵ and 1 x 10⁶ viable cells/mL. Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Cryopreservation	Complete growth medium described above supplemented with 5% (v/v) DMSO. Cell culture tested DMSO is available as ATCC Catalog No. 4-X.
Culture Conditions	Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0°C
Isotype	IgG1 kappa
Name of Depositor	J Dean
Deposited As	mouse (B cell); mouse (myeloma)
References	Animal were immunized with bacterially expressed, Hi2+-agarose purified 6-histidine tagged recombinant (amino acids 39-642) human ZP2 (zona pellucida glycoprotein 2) Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online.

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