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HEC-1-B

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編號:B164587

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產(chǎn)品名稱	HEC-1-B
商品貨號	B164587
Organism	Homo sapiens, human
Tissue	uterus; endometrium
Product Format	frozen
Morphology	epithelial
Culture Properties	adherent
Biosafety Level	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease	adenocarcinoma
Age	71 years
Gender	female
Applications	This cell line is a suitable transfection host.
Karyotype	diploid to tetraploid with large submetacentric marker
Derivation	This is a substrain of HEC-1-A (see ATCC HTB-112) isolated in 1968 by H. Kuramoto.
Clinical Data	female
Antigen Expression	Blood Type B; Rh+
Tumorigenic	Yes
Effects	Yes, in nude mice (The cells form moderately well differentiated adenocarcinomas consistent with endometrial carcinoma (grade II).) Yes, in steroid-treated hamsters
Comments	This is a substrain of HEC-1-A. Unlike HEC-1-A, this substrain exhibited a stationary growth period between the 135th and 190th days in culture and appeared on recovery to be flattened and more pavement patterned than the parent line.The predominant complement of chromosomes was double that observed for the parent line.
Complete Growth Medium	The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing	Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 mL of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37°C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks. Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended Medium Renewal: Every 2 to 3 days
Cryopreservation	Culture medium, 95%; DMSO, 5%
Culture Conditions	Temperature: 37°C
STR Profile	Amelogenin: X CSF1PO: 10,12 D13S317: 11,16 D16S539: 11,12 D5S818: 11,13 D7S820: 9,11 THO1: 6,7 TPOX: 8,11 vWA: 18
Isoenzymes	AK-1, 1 ES-D, 1 G6PD, B GLO-I, 2 Me-2, 2 PGM1, 1 PGM3, 1-2
Name of Depositor	H Kuramoto
Deposited As	Homo sapiens
References	Kuramoto H. Studies of the growth and cytogenetic properties of human endometrial adenocarcinoma in culture and its development into an established line. Acta Obstet. Gynaecol. Jpn. 19: 47-58, 1972. PubMed: 4678779 Fogh J, et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214, 1977. PubMed: 833871 Goodfellow M, et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J. Natl. Cancer Inst. 59: 221-226, 1977. PubMed: 77210034 Presta M, et al. Modulation of plasminogen activator activity in human endometrial adenocarcinoma cells by basic fibroblast growth factor and transforming growth factor beta. Cancer Res. 48: 6384-6389, 1988. PubMed: 3263185 Kuramoto H, et al. Establishment of a cell line of human endometrial adenocarcinoma in vitro. Am. J. Obstet. Gynecol. 114: 1012-1019, 1972. PubMed: 4673779 St. Geme JW, et al. Characterization of the genetic locus encoding Haemophilus influenzae type b surface fibrils. J. Bacteriol. 178: 6281-6287, 1996. PubMed: 8892830 Schramm N, et al. Vesicles containing Chlamydia trachomatis serovar L2 remain above pH 6 within HEC-1B cells. Infect. Immun. 64: 1208-1214, 1996. PubMed: 8606080 The cells form moderately well differentiated adenocarcinomas consistent with endometrial carcinoma (grade II).
Cross References	Nucleotide (GenBank) : AF004337 Homo sapiens 7S ribosomal RNA, mitochondrial gene, partial sequence.

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