| 產(chǎn)品名稱(chēng) | HeLa S3 |
|---|---|
| 商品貨號(hào) | B164592 |
| Organism | Homo sapiens, human |
| Tissue | cervix |
| Product Format | frozen |
| Morphology | epithelial |
| Culture Properties | adherent |
| Biosafety Level | 2 [Cells contain human papilloma virus (HPV-18)]
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease | adenocarcinoma |
| Age | 31 years |
| Gender | female |
| Ethnicity | Black |
| Applications | This cell line is a suitable transfection host. The HeLa S3 clone has been very useful in the clonal analysis of mammalian cell populations relating to chromosomal variation, cell nutrition, and plaque-forming ability. |
| Storage Conditions | liquid nitrogen vapor phase |
| Karyotype | A medium-sized metacentric marker is present in 100% of the cells. HeLa Markers: One copy of M1, one copy of M2, two copies of M3, and one copy of M4. Note: Cytogenetic information is based on initial seed stock at ATCC. Cytogenetic instability has been reported in the literature for some cell lines. |
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| Derivation | HeLa S3 is a clonal derivative of the parent HeLa line (see ATCC CCL-2). S3 was cloned in 1955 by T.T. Puck, P.I. Marcus, and S.J. Cieciura. |
| Clinical Data | 31 years Black female |
| HeLa Markers | Y |
| Genes Expressed | The cells are positive for keratin by immunoperoxidase staining.
keratin. |
| Cellular Products | keratin |
| Virus Susceptibility | Vesicular stomatitis, Glasgow (Indiana)
Vesicular stomatitis, Orsay (Indiana) Encephalomyocarditis virus Human adenovirus 5 |
| Virus Resistance | Human poliovirus 1 Human poliovirus 2 Human poliovirus 3 |
| Comments | This line can be adapted to grow in suspension.
The cells are positive for keratin by immunoperoxidase staining. A culture at approximately passage 400 was submitted to the American Type Culture Collection in February, 1972. HeLa cells have been reported to contain human papilloma virus 18 (HPV-18) sequences. ATCC confirmed this cell line is positive for the presence of Papillomavirus viral DNA sequences via PCR. |
| Complete Growth Medium | The base medium for this cell line is ATCC-formulated F-12K Medium, Catalog No. 30-2004. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing | Volumes used in this protocol are for 75 cm2 flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. Flasks do not become 100% confluent. Cells are rounded and have a tendency to float in the medium.
Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:10 is recommended
Medium Renewal: 2 to 3 times per week |
| Cryopreservation | Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions | Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37°C |
| STR Profile | Amelogenin: X CSF1PO: 9,10 D13S317: 13.3 D16S539: 9,10 D5S818: 11,12 D7S820: 8,12 THO1: 7 TPOX: 8,12 vWA: 16,18 |
| Isoenzymes | G6PD, A |
| Name of Depositor | TT Puck |
| Deposited As | Homo sapiens |
| Passage History | A culture at approximately passage 400 was submitted to the American Type Culture Collection in February, 1972. |
| Year of Origin | 1955 |
| References | Chen TR. Re-evaluation of HeLa, HeLa S3, and HEp-2 karyotypes. Cytogenet. Cell Genet. 48: 19-24, 1988. PubMed: 3180844 Boshart M, et al. A new type of papillomavirus DNA, its presence in genital cancer biopsies and in cell lines derived from cervical cancer. EMBO J. 3: 1151-1157, 1984. PubMed: 6329740 Puck TT, et al. Clonal growth of mammalian cells in vitro; growth characteristics of colonies from single HeLa cells with and without a feeder layer. J. Exp. Med. 103: 273-283, 1956. PubMed: 13286432 Yee C, et al. Presence and expression of human papillomavirus sequences in human cervical carcinoma cell lines. Am. J. Pathol. 119: 361-366, 1985. PubMed: 2990217 Puck TT, Marcus PI. A rapid method for viable cell titration and clone production with HeLa cells in tissue culture: the use of x-irradiated cells to supply conditioning factors. Proc. Natl. Acad. Sci. USA 41: 432-437, 1955. Puck TT, Fisher HW. Genetics of Somatic Mammalian Cells. J. Exp. Med. 104: 427-434, 1956. PubMed: 19867118 Ham RG, Puck TT. Quantitative colonial growth of isolated mammalian cells. Methods Enzymol 5 90-119 1962 Darnell JE Jr., et al. The effect of cell population density on the amino acid requirements for poliovirus synthesis in HeLa cells. J. Exp. Med. 110: 445-450, 1959. PubMed: 13814142 Cohen EP, Eagle H. A simplified chemostat for the growth of mammalian cells: characteristics of cell growth in continuous culture. J. Exp. Med. 113: 467-474, 1961. Darnell JE Jr., Sawyer TK. Variation in plaque-forming ability among parental and clonal strains of HeLa cells. Virology 8: 223-229, 1959. PubMed: 13669339 Sato G, et al. Molecular growth requirements of single mammalian cells. Science 126: 461-464, 1957. PubMed: 13486039 Soares K, et al. cis-Acting elements involved in transcriptional regulation of the herpes simples virus type 1 latency-associated promoter 1 (LAP1) in vitro and in vivo. J. Virol. 70: 5384-5394, 1996. PubMed: 8764049 Chang YE, et al. Properties of the protein encoded by the UL32 open reading frame of herpes simplex virus 1. J. Virol. 70: 3938-3946, 1996. PubMed: 8648731 Jiang BH, et al. Hypoxia-inducible factor 1 levels vary exponentially over a physiologically relevant range of O2 tension. Am. J. Physiol. 271: C1172-C1180, 1996. PubMed: 8897823 Genuario RR, Perry RP. The GA-binding protein can serve as both an activator and repressor of ribosomal protein gene transcription. J. Biol. Chem. 271: 4388-4395, 1996. PubMed: 8626789 Ladner RD, et al. Identification of a consensus cyclin-dependent kinase phosphorylation site unique to the nuclear form of human deoxyuridine triphosphate nucleotidohydrolase. J. Biol. Chem. 271: 7752-7757, 1996. PubMed: 8631817 Ladner RD, et al. Characterization of distinct nuclear and mitochondrial forms of human deoxyuridine triphosphate nucleotidohydrolase. J. Biol. Chem. 271: 7745-7751, 1996. PubMed: 8631816 Stewart L, et al. Biochemical and biophysical analyses of recombinant forms of human topoisomerase I. J. Biol. Chem. 271: 7593-7601, 1996. PubMed: 8631793 |
| Cross References | Nucleotide (GenBank) : Y09723 H.sapiens mRNA for Miz-1 protein. Nucleotide (GenBank) : Y08698 H.sapiens mRNA for RanBP3 (59 kDa). Nucleotide (GenBank) : Y08699 H.sapiens mRNA for RanBP3, splice variant. Nucleotide (GenBank) : U25182 Human antioxidant enzyme AOE37-2 mRNA, complete cds. Nucleotide (GenBank) : U33822 Human tax1-binding protein TXBP181 mRNA, complete cds. Nucleotide (GenBank) : U43430 Human epsilon isoform 14-3-3 protein mRNA, complete cds. Nucleotide (GenBank) : Y08697 H.sapiens mRNA for RanBP3 small splice variant (53 kDa). Nucleotide (GenBank) : L15328 Saccharomyces cerevisiae RNA helicase gene, complete cds. Nucleotide (GenBank) : U33821 Homo sapiens tax1-binding protein TXBP151 mRNA, complete cds. Nucleotide (GenBank) : NM_003443 Homo sapiens zinc finger protein 151 (pHZ-67) (ZNF151), mRNA. Nucleotide (GenBank) : AF034102 Homo sapiens NBMPR-insensitive nucleoside transporter ei (ENT2) mRNA, complete cds. Nucleotide (GenBank) : NM_006024 Homo sapiens Tax1 (human T-cell leukemia virus type I) binding protein 1 (TAX1BP1), mRNA. |
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