| 產品名稱 | Het-1A |
|---|---|
| 商品貨號 | B164599 |
| Organism | Homo sapiens, human |
| Tissue | esophagus |
| Cell Type | epithelial; SV40 large T antigen transfected |
| Product Format | frozen |
| Morphology | epithelial |
| Culture Properties | adherent |
| Biosafety Level | 2 [cells contain SV40 viral sequences]
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease | normal |
| Age | 25 years |
| Gender | male |
| Ethnicity | Black |
| Applications | The cell line may be useful for investigating the action of putative esophageal carcinogens. |
| Storage Conditions | liquid nitrogen vapor phase |
| Karyotype | hypodiploid (34-40 chromosomes)
Ref  Stoner GD, et al. Establishment and characterization of SV40 T-antigen immortalized human esophageal epithelial cells. Cancer Res. 51: 365-371, 1991. PubMed: 1703038 |
| Derivation | The HET-1A cell line was derived in 1986 from human esophageal autopsy tissue by transfection with plasmid pRSV-T consisting of the RSV-LTR promoter and the sequence encoding the simian virus 40 large T-antigen. |
| Clinical Data | 25 years
Black
male |
| Antigen Expression | SV40 T antigen Ref Stoner GD, et al. Establishment and characterization of SV40 T-antigen immortalized human esophageal epithelial cells. Cancer Res. 51: 365-371, 1991. PubMed: 1703038RefTolleson WH, et al. The mycotoxin fumonisin induces apoptosis in cultured human cells and in livers and kidneys of rats. Adv. Exp. Med. Biol. 392: 237-250, 1996. PubMed: 8850621 |
| Genes Expressed | cytokeratin Ref Stoner GD, et al. Establishment and characterization of SV40 T-antigen immortalized human esophageal epithelial cells. Cancer Res. 51: 365-371, 1991. PubMed: 1703038 |
| Cellular Products | cytokeratin Ref Stoner GD, et al. Establishment and characterization of SV40 T-antigen immortalized human esophageal epithelial cells. Cancer Res. 51: 365-371, 1991. PubMed: 1703038 |
| Tumorigenic | No |
| Effects | No, nontumorigenic in athymic, nude mice for more than 12 months. Ref Stoner GD, et al. Establishment and characterization of SV40 T-antigen immortalized human esophageal epithelial cells. Cancer Res. 51: 365-371, 1991. PubMed: 1703038 |
| Comments | The line has undergone more than 250 population doublings. Growth factor studies have shown that HET-1A is stimulated by calcium and inhibited by fetal bovine serum, transforming growth factor-beta 1, and transforming growth factor-beta 2. Fumonisin B1 produces growth inhibition and increased apoptosis in HET-1A cells. The synthetic retinoid CD437 (6-[3-(1-adamantyl)-4-hydroxyphenyl]-2-naphthalene carboxylic acid (AHPN/CD437)0 induces apoptosis of esophageal squamous HET-1A cells through the caspase-3-dependent pathway. |
| Complete Growth Medium | The base medium for this cell line (BEBM) along with all the additives can be obtained from Lonza/Clonetics Corporation as a kit: BEGM, Kit Catalog No. CC-3170. ATCC does not use the GA-1000 (gentamycin-amphotericin B mix) provided with the BEGM kit. Note: Do not filter complete medium.
|
| Subculturing | The flasks used should be precoated with a mixture of 0.01 mg/mL fibronectin, 0.03 mg/mL bovine collagen type I and 0.01 mg/mL bovine serum albumin dissolved in culture medium.
Subcultivation ratio: 1:3 to 1:4 Medium Renewal: Every 2 to 3 days.
Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in Culture of Animal Cells, a manual of Basic Technique by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994.
|
| Cryopreservation | Freeze medium: Leibovitz's L-15 medium with 2 mM L-glutamine and 10mM HEPES supplemented with 1% PVP, 10% fetal bovine serum and 7.5% DMSO Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions | Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C |
| STR Profile | Amelogenin: X,Y CSF1PO: 10,12 D13S317: 11 D16S539: 9,11 D5S818: 11,12 D7S820: 9 THO1: 7 TPOX: 11 vWA: 16 |
| Name of Depositor | CC Harris |
| Deposited As | human |
| Year of Origin | 1986 |
| References | Stoner GD, et al. Establishment and characterization of SV40 T-antigen immortalized human esophageal epithelial cells. Cancer Res. 51: 365-371, 1991. PubMed: 1703038 Milo GE, et al. Conversion of premalignant human cells to tumorigenic cells by methylmethane sulfonate and methylnitronitrosoguanidine. Cell Biol. Toxicol. 8: 193-205, 1992. PubMed: 1337307 Tolleson WH, et al. Apoptotic and anti-proliferative effects of fumonisin B1 in human keratinocytes, fibroblasts, esophageal epithelial cells and hepatoma cells. Carcinogenesis 17: 239-249, 1996. PubMed: 8625445 Tolleson WH, et al. The mycotoxin fumonisin induces apoptosis in cultured human cells and in livers and kidneys of rats. Adv. Exp. Med. Biol. 392: 237-250, 1996. PubMed: 8850621 Wan X, et al. Synthetic retinoid CD437 induces apoptosis of esophageal squamous HET-1A cells through the caspase-3-dependent pathway. Anticancer Res. 21: 2657-2663, 2001. PubMed: 11724335 Stoner GD, et al. Establishment and characterization of SV40 T-antigen immortalized human esophageal epithelial cells. Cancer Res. 51: 365-371, 1991. PubMed: 1703038 |
| 梅經理 | 17280875617 | 1438578920 |
| 胡經理 | 13345964880 | 2438244627 |
| 周經理 | 17757487661 | 1296385441 |
| 于經理 | 18067160830 | 2088210172 |
| 沈經理 | 19548299266 | 2662369050 |
| 李經理 | 13626845108 | 972239479 |
