| 產品名稱 |
HFN 36.3 |
| 商品貨號 |
B164602 |
| Organism |
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Tissue |
spleen |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications |
The antibody cross-reacts with fibronectins from 26 species including 15 primates. Tested and found negative for ectromelia virus (mousepox). |
| Derivation |
Mice were immunized with purified human fibronectin. Spleen cells were fused with P3X63Ag8 myeloma cells. |
| Antigen Expression |
H-2d |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against human fibronectin,H-2d |
| Cellular Products |
immunoglobulin; monoclonal antibody; against human fibronectin |
| Comments |
The antibody cross-reacts with fibronectins from 26 species including 15 primates. Mice were immunized with purified human fibronectin. Spleen cells were fused with P3X63Ag8 myeloma cells. Tested and found negative for ectromelia virus (mousepox). Formerly ATCC HB-40. |
| Complete Growth Medium |
Dulbecco's modified Eagle's medium, 90%; newborn bovine calf serum, 10%
|
| Subculturing |
Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1 X 10 exp5 viable cells/ml and subculture at 1 X 10 exp6 cells/ml. |
| Isotype |
IgG1 |
| Name of Depositor |
RC Schoen, KL Bentley, RJ Klebe |
| Deposited As |
mouse (B cell); mouse (myeloma) |
| References |
Schoen RC, et al. Monoclonal antibody against human fibronectin which inhibits cell attachment. Hybridoma 1: 99-108, 1982. PubMed: 6208125
|
