| 產品名稱 |
HO-22-1 |
| 商品貨號 |
B164625 |
| Organism |
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications |
Tested and found negative for ectromelia virus (mousepox). |
| Derivation |
Spleen cells were fused with P3X63Ag8 myeloma cells. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against Thy-1.1 antigen |
| Cellular Products |
immunoglobulin; monoclonal antibody; against Thy-1.1 antigen |
| Comments |
Animals were immunized with AKR/J thymocytes. Spleen cells were fused with P3X63Ag8 myeloma cells. Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium |
Dulbecco's modified Eagle's medium, with non-essential amino acids, 80%; fetal bovine serum, 20%
|
| Subculturing |
Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 5 x 10 exp4 cells/ml and maintain between 1 x 10 exp5 and 1 x 10 exp6 cells/ml. |
| Isotype |
IgM |
| Name of Depositor |
A Marshak-Rothstein |
| Deposited As |
mouse (B cell); mouse (myeloma) |
| References |
Marshak-Rothstein A, et al. Properties and applications of monoclonal antibodies directed against determinants of the Thy-1 locus. J. Immunol. 122: 2491-2497, 1979. PubMed: 87465
|
