青青草原av-午夜视频免费看-免费久久久-中国av片-欧美一级欧美三级在线观看-火影忍者羞羞漫画-成av人片在线观看www-国产日本亚洲-欧美视频在线免费-日本韩国在线-在线日韩中文字幕-国产成人三级在线播放-久久福利在线-老司机免费精品视频-男人操女人逼逼视频-av大片免费-欧美精品第二页-操校花视频-欧美插插视频-优优色综合

• 0.05 mM 2-mercaptoethanol
• fetal bovine serum to a final concentration of 10%

Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 x 10⁵ viable cells/mL.  Maintain cultures at a cell concentration between 1 x 10⁵ and 1 x 10⁶ cells/mL.

Complete growth medium described above supplemented with 5% (v/v) DMSO.  Cell culture tested DMSO is available as ATCC Catalog No. 4-X.

Reimer CB, et al. Evaluation of thirty-one mouse monoclonal antibodies to human IgG epitopes. Hybridoma 3: 263-275, 1984. PubMed: 6209201

Jefferis R, et al. Evaluation of monoclonal antibodies having specificity for human IgG sub-classes: Results of an IUIS/WHO collaborative study. Immunol. Lett. 10: 223-252, 1985. PubMed: 3899923

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.