| 產品名稱 |
Hs 913T |
| 商品貨號 |
B164773 |
| Organism |
Homo sapiens, human |
| Tissue |
Derived from metastatic site: lung |
| Product Format |
frozen |
| Morphology |
fibroblast |
| Culture Properties |
adherent |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease |
fibrosarcoma |
| Age |
51 years |
| Gender |
male |
| Ethnicity |
Caucasian |
| Karyotype |
modal number = 111 to 113; range = 95 to 120; large submetacentric and double minute (DM) markers |
| Clinical Data |
male Caucasian 51 years |
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing |
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended Medium Renewal: 2 to 3 times per week Remove medium, rinse with fresh 0.25% trypsin, 0.02% EDTA solution, remove trypsin and let the culture sit at room temperature (or at 37C) until the cells detach (about 10 minutes). Add fresh medium, aspirate and dispense into new flasks. Subculture every 6 to 8 days. |
| Cryopreservation |
Culture medium, 95%; DMSO, 5% |
| STR Profile |
Amelogenin: X,Y CSF1PO: 12 D13S317: 11 D16S539: 13 D5S818: 10 D7S820: 10 THO1: 8,9.3 TPOX: 9,11 vWA: 17,18 |
| Isoenzymes |
AK-1, 1 ES-D, 1 G6PD, B GLO-I, 1 PGM1, 2 PGM3, 2 |
| Name of Depositor |
RB Owens |
| Deposited As |
Homo sapiens |
