| 產品名稱 |
HT-1376 |
| 商品貨號 |
B164799 |
| Organism |
Homo sapiens, human |
| Tissue |
urinary bladder |
| Product Format |
frozen |
| Morphology |
epithelial |
| Culture Properties |
adherent |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease |
grade 3, carcinoma |
| Age |
58 years |
| Gender |
female |
| Ethnicity |
Caucasian |
| Applications |
This cell line is a suitable transfection host. |
| Storage Conditions |
liquid nitrogen vapor phase |
| Karyotype |
modal chromosome number = 104 to 121 |
| Clinical Data |
58 years Caucasian female |
| Genes Expressed |
fibrinolytic activity; interferon |
| Cellular Products |
fibrinolytic activity; interferon |
| Tumorigenic |
Yes |
| Effects |
Yes, in mice and hamsters |
| Comments |
The line does not contain detectable virus particles, herpesvirus antigens or retrovirus antigens. The cells will grow in soft agar. |
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing |
Remove medium, add fresh 0.25% trypsin, 0.02% EDTA solution, rinse and remove. Allow culture to sit at room temperature or 37°C until cells detach (about 10 minutes), add fresh medium, aspirate and dispense into new flasks. Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended Medium Renewal: Every 2 to 3 days |
| Cryopreservation |
Freeze Medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage Temperature: Liquid nitrogen vapor phase |
| Culture Conditions |
Atmosphere: Air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C |
| STR Profile |
Amelogenin: X CSF1PO: 12 D13S317: 9,11 D16S539: 11,14 D5S818: 11,12 D7S820: 9,12 THO1: 7,10 TPOX: 8 vWA: 15,18 |
| Isoenzymes |
G6PD, B |
| Name of Depositor |
S Rasheed |
| Deposited As |
Homo sapiens |
| References |
Rasheed S, et al. Human bladder carcinoma: characterization of two new tumor cell lines and search for tumor viruses. J. Natl. Cancer Inst. 58: 881-890, 1977. PubMed: 191628
Jameson P, Grossberg SE. Production of interferon by human tumor cell lines. Arch. Virol. 62: 209-219, 1979. PubMed: 229805
Bellet D, et al. Malignant transformation of nontrophoblastic cells is associated with the expression of chorionic gonadotropin beta genes normally transcribed in trophoblastic cells. Cancer Res. 57: 516-523, 1997. PubMed: 9012484
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