| 產品名稱 |
I1-Hybridoma |
| 商品貨號 |
B164820 |
| Organism |
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Tissue |
spleen |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease |
Vesicular Stomatitis |
| Age |
adult |
| Applications |
The antibody reacts with the major surface glycoprotein (G-protein) of vesicular stomatitis virus, Indiana Serotype (VSV-Ind. |
| Storage Conditions |
liquid nitrogen vapor phase |
| Derivation |
Spleen cells were fused with Sp2/0-Ag14 mouse myeloma cells. |
| Tumorigenic |
Yes |
| Effects |
Yes, intraperitoneal injection into BALB/c or (BALB/c X BALB.B)F1 mice |
| Comments |
Animals were immunized with vesicular stomatitis virus, Indiana Serotype (VSV-Ind.). Spleen cells were fused with Sp2/0-Ag14 mouse myeloma cells. The antibody reacts with the major surface glycoprotein (G-protein) of vesicular stomatitis virus, Indiana Serotype (VSV-Ind.). |
| Complete Growth Medium |
Minimum essential medium (Eagle) with 2.0 mM L-glutamine and Hanks' BSS adjusted to contain 0.35 g/L sodium bicarbonate 80%; fetal bovine serum, 20%.
|
| Subculturing |
Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2 X 10(5) viable cells/ml. Maintain cell density between 1 X 10(5) and 1 X 10(6) viable cells/ml.
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Note: This medium is formulated for use in a free gas exchange with atmospheric air. A 5% CO2 in air atmosphere is detrimental to cells when using this medium for culturing. |
| Cryopreservation |
Freeze medium: Complete growth medium supplemented with an additional 45% fetal bovine serum and 10% DMSO
Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions |
Temperature: 37°C
Atmosphere: Air, 100% |
| Isotype |
mouse IgG2a kappa |
| Name of Depositor |
IS Novella, DS Lyles |
| Deposited As |
Mus musculus (B cell); Mus musculus (myeloma) |
| References |
Lefrancios L, Lyles DS. The interaction of antibody with the major surface glycoprotein of vesicular stomatitis virus. I. Analysis of neutralizing epitopes with monoclonal antibodies. Virology 121: 157-167, 1982. PubMed: 6180550
|
