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IgG-1B3 [anti LRP 85]

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編號:B164838

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產品名稱	IgG-1B3 [anti LRP 85]
商品貨號	B164838
Organism	Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type	hybridoma: B lymphocyte
Product Format	frozen
Morphology	lymphoblast
Culture Properties	suspension
Biosafety Level	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Applications	The antibody reacts specifically with the 85 kDa subunit of rabbit LRP. It cross-reacts weakly with the 85 kDa subunit of rat LRP.
Storage Conditions	liquid nitrogen vapor phase
Derivation	Animals were immunized with purified rabbit LRP. Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
Genes Expressed	immunoglobulin; monoclonal antibody; against the 85000 dalton (85 kDa) subunit of rabbit lipoprotein receptor related protein (LRP)
Cellular Products	immunoglobulin; monoclonal antibody; against the 85000 dalton (85 kDa) subunit of rabbit lipoprotein receptor related protein (LRP)
Comments	Animals were immunized with purified rabbit LRP. Spleen cells were fused with Sp2/0-Ag14 myeloma cells. The antibody reacts specifically with the 85 kDa subunit of rabbit LRP. It cross-reacts weakly with the 85 kDa subunit of rat LRP. The antibody does not react with the 515 kDa subunit of rabbit LRP.
Complete Growth Medium	The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing	Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2 x 10⁵ viable cells/mL. Maintain cell density between 1 x 10⁵ and 1 x 10⁶ viable cells/mL. Medium Renewal: Two to three times weekly
Cryopreservation	Complete growth medium described above supplemented with 5% (v/v) DMSO. Cell culture tested DMSO is available as ATCC Catalog No. 4-X.
Culture Conditions	Temperature: 37°C Atmosphere: Air, 95%; Carbon dioxide (CO₂), 5%
Isotype	IgG1
Name of Depositor	YK Ho, JL Goldstein
Deposited As	mouse (B cell); mouse (myeloma)
References	Herz J, et al. Low density lipoprotein receptor-related protein mediates endocytosis of monoclonal antibodies in cultured cells and rabbit liver. J. Biol. Chem. 265: 21355-21362, 1990. PubMed: 2250029 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

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