| 產(chǎn)品名稱 |
ILB1-H34 [ILB1-H34A3] |
| 商品貨號(hào) |
B164854 |
| Organism |
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications |
The antibody reacts specifically with IL-1 beta and does not react with IL-1 alpha or with acidic or basic fibroblast growth factor. |
| Disclosure |
This material is cited in a US or other Patent and may not be used to
infringe the claims. Depending on the wishes of the Depositor, ATCC may be
required to inform the Patent Depositor of the party to which the material was
furnished. This material may not have been produced or characterized by ATCC. |
| Derivation |
Animals were immunized with purified recombinant human IL-1 beta (170500 dalton form). Spleen cells were fused with P3X63Ag8.653 myeloma cells. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against human interleukin 1 beta (interleukin-1 beta, IL-1 beta) |
| Cellular Products |
immunoglobulin; monoclonal antibody; against human interleukin 1 beta (interleukin-1 beta, IL-1 beta) |
| Tumorigenic |
Yes |
| Effects |
Yes, forms ascites in BALB/c mice |
| Comments |
Animals were immunized with purified recombinant human IL-1 beta (170500 dalton form). Spleen cells were fused with P3X63Ag8.653 myeloma cells. The antibody reacts specifically with IL-1 beta and does not react with IL-1 alpha or with acidic or basic fibroblast growth factor. It recognizes an epitope distinct from that bound by ILB1-H21 and ILB1-H67 (ATCC HB-10220 and ATCC HB-10222) and that may overlap the epitope bound by ILB1-H6 (ATCC HB-10219). The affinity constant of ILB1-H34 was measured to be 3.3 X 10 exp10 liter/mol. |
| Complete Growth Medium |
The base medium for this cell line is ATCC Hybri-Care Medium, Catalog No. 46-X. Hybri-Care Medium is supplied as a powder and should be reconstituted in 1 L cell-culture-grade water and supplemented with 1.5 g/L sodium bicarbonate. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing |
Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Cryopreservation |
culture medium, 90%; DMSO, 10% |
| Isotype |
IgG1; kappa light chain |
| Name of Depositor |
Syntex (USA), Inc. |
| Deposited As |
mouse (B cell); mouse (myeloma) |
| U.S. Patent Number |
|
| References |
Allison AC, et al. Monoclonal antibodies for interleukin-1.beta. US Patent 4,935,343 dated Jun 19 1990
Kenney JS, et al. Monoclonal antibodies to human recombinant interleukin 1 (IL 1)beta: quantitation of IL 1 beta and inhibition of biological activity. J. Immunol. 138: 4236-4242, 1987. PubMed: 2438338
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