| 產品名稱 | IMR-32 |
|---|---|
| 商品貨號 | B164859 |
| Organism | Homo sapiens, human |
| Tissue | brain; derived from metastatic site: abdominal mass |
| Cell Type | neuroblast |
| Product Format | frozen |
| Morphology | fibroblast; neuroblast |
| Culture Properties | adherent |
| Biosafety Level | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease | neuroblastoma |
| Age | 13 months |
| Gender | male |
| Ethnicity | Caucasian |
| Applications | This cell line is a suitable transfection host. |
| Storage Conditions | liquid nitrogen vapor phase |
| Karyotype | Stable male karyotype with stemline number of 49. Two large marker chromosomes with submedian centromeres. A deletion in one number 1 chromosome: One number 16 chromosome missing; two extra chromosomes in C group. Sublines with 50 and 48 chromosomes differ from those with 49 chromosomes by having an extra or missing C group chromosome respectively. Note: Cytogenetic information is based on initial seed stock at ATCC. Cytogenetic instability has been reported in the literature for some cell lines. |
| Images |  |
| Derivation | The IMR-32 cell line was established by W.W. Nichols, J. Lee and S. Dwight in April, 1967 from an abdominal mass occurring in a 13-month-old Caucasian male. The tumor was diagnosed as a neuroblastoma with rare areas of organoid differentiation. |
| Clinical Data | 13 months
Caucasian
male
|
| Virus Susceptibility | Vesicular stomatitis, Orsay (Indiana)
Vesicular stomatitis, Glasgow (Indiana) Herpes simplex virus Vaccinia virus Human Coxsackievirus B3 Human poliovirus 3 |
| Virus Resistance | {8B5AD8F2-1676-4399-B5A1-85F54726A74D} |
| Comments | Two cell types are present. Predominant is a small neuroblast-like cell.The other is a large hyaline fibroblast.
IMR-32 cells may pile up and grow in patches. IMR-32 cells may not become 100% confluent. |
| Complete Growth Medium | The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing | Volumes used in this protocol are for 75 cm2 flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended
Medium Renewal: Every 2 to 3 days |
| Cryopreservation | Freeze medium: Complete growth medium 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor temperature |
| Culture Conditions | Temperature: 37°C |
| STR Profile | Amelogenin: X,Y CSF1PO: 11,12 D13S317: 9 D16S539: 8 D5S818: 11,12 D7S820: 9,10 THO1: 7,9.3 TPOX: 11 vWA: 15 |
| Isoenzymes | G6PD, B |
| Population Doubling Time | approximately 20 hrs. |
| Name of Depositor | WW Nichols |
| Deposited As | Homo sapiens |
| Passage History | The cell line was submitted to the American Type Culture Collection in the 36th passage. It has been demonstrated that the cells can be propagated successfully beyond the 80th serial subculture. |
| Year of Origin | April, 1967 |
| References | Tumilowicz JJ, et al. Definition of a continuous human cell line derived from neuroblastoma. Cancer Res. 30: 2110-2118, 1970. PubMed: 5459762 Rostomily RC, et al. Expression of neurogenic basic helix-loop-helix genes in primitive neuroectodermal tumors. Cancer Res. 57: 3526-3531, 1997. PubMed: 9270024 Maestrini E, et al. A family of transmembrane proteins with homology to the MET-hepatocyte growth factor receptor. Proc. Natl. Acad. Sci. USA 93: 674-678, 1996. PubMed: 8570614 |
| 梅經理 | 17280875617 | 1438578920 |
| 胡經理 | 13345964880 | 2438244627 |
| 周經理 | 17757487661 | 1296385441 |
| 于經理 | 18067160830 | 2088210172 |
| 沈經理 | 19548299266 | 2662369050 |
| 李經理 | 13626845108 | 972239479 |
