| 產(chǎn)品名稱 |
intestine 407 |
| 商品貨號(hào) |
B164862 |
| Organism |
Homo sapiens, human |
| Tissue |
HeLa contaminant |
| Product Format |
frozen |
| Morphology |
epithelial |
| Culture Properties |
adherent |
| Biosafety Level |
2 [Cells contain human papilloma virus (HPV-18)]
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Gender |
female |
| Storage Conditions |
liquid nitrogen vapor phase |
| Karyotype |
NOTE: Cells of this line contain HeLa marker chromosomes, and were derived via HeLa contamination. HeLa Marker Chromosomes: One copy of M1, one copy of M2, four copies of M3 and one copy of M4 as revealed by G-banding patterns. |
| Derivation |
This line was originally thought to be derived from normal embryonic intestinal tissue, but was subsequently found, based on isoenzyme analysis, HeLa marker chromosomes, and DNA fingerprinting, to have been established via HeLa cell contamination. |
| Clinical Data |
female |
| HeLa Markers |
Y |
| Genes Expressed |
keratin |
| Cellular Products |
keratin |
| Tumorigenic |
Yes |
| Effects |
Yes, in hamster (untreated) cheek pouch Yes, in immunosuppressed rats |
| Virus Susceptibility |
Human poliovirus 1
Human poliovirus 2
Human poliovirus 3
Vesicular stomatitis, Orsay (Indiana)
Vesicular stomatitis, Glasgow (Indiana)
Human adenovirus 3
Herpes simplex virus
Pseudorabies virus
Vaccinia virus
Human coxsackievirus B1
Human Coxsackievirus B 2
Human Coxsackievirus B3
Human Coxsackievirus B4
Human Coxsackievirus B 5
Human Coxsackievirus B 6
Mumps rubulavirus
,
Mumps virus
Newcastle disease virus
|
| Virus Resistance |
rabiesvirus |
| Comments |
The cells are positive for keratin by immunoperoxidase staining
Cells contain human papilloma virus (HPV-18).
|
| Complete Growth Medium |
Basal medium (Eagle) in Earle's BSS, 90%; fetal bovine serum, 10%
|
| Subculturing |
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
- Remove and discard culture medium.
-
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
-
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach.
Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
-
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
-
Add appropriate aliquots of the cell suspension to new culture vessels.
- Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:8 is recommended
Medium Renewal: Every 2 to 3 days |
| Cryopreservation |
Freeze Medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage Temperature: liquid nitrogen vapor phase |
| Culture Conditions |
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C |
| STR Profile |
Amelogenin: X CSF1PO: 9,10 D13S317: 12,13.3 D16S539: 9,10 D5S818: 11,12 D7S820: 8,12 THO1: 7 TPOX: 8,12 vWA: 16,18 |
| Isoenzymes |
G6PD, A |
| Name of Depositor |
G Henle, F Deinhardt |
| Deposited As |
Homo sapiens |
| Year of Origin |
January, 1955 |
| References |
Henle G, Deinhardt F. The establishment of strains of human cells in tissue culture. J. Immunol. 79: 54-59, 1957. PubMed: 13475808
Lavappa KS. Survey of ATCC stocks of human cell lines for HeLa contamination. In Vitro 14: 469-475, 1978. PubMed: 566722
Lavappa KS, et al. Examination of ATCC stocks for HeLa marker chromosomes in human cell lines. Nature 259: 211-213, 1976. PubMed: 1250349
St. Geme JW, et al. Characterization of the genetic locus encoding Haemophilus influenzae type b surface fibrils. J. Bacteriol. 178: 6281-6287, 1996. PubMed: 8892830
White LJ, et al. Attachment and entry of recombinant norwalk virus capsids to cultured human and animal cell lines. J. Virol. 70: 6589-6597, 1996. PubMed: 8794293
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