| 產(chǎn)品名稱 |
J.gamma1 |
| 商品貨號(hào) |
B164867 |
| Organism |
Homo sapiens, human |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
clusters in suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease |
acute T cell leukemia |
| Gender |
male |
| Derivation |
Wild-type Jurkat cells were mutagenized with the frameshifting mutagen ICR-191 and clones were isolated that failed to increase calcium in response to pervanadate. The selection procedure was repeated on the P98 clone to establish the J.gamma1 cell line. |
| Clinical Data |
male |
| Comments |
The J.gamm1 cell line is a phospholipase C-gamma1 (PLC-gamma1) deficient mutant of the E6-1 clone of Jurkat (ATCC TIB-152).
The J.gamma1.WT derivative (ATCC CRL-2679) stably expresses PLC-gamma1at levels comparable to the Jurkat cell line.
The J.gamma1 subline contains no detectable PLC-gamma1 protein. The lack of PLC-gamma1 expression in J.gamma1 cells causes profound defects in T cell receptor (TCR) calcium mobilization and nuclear factor of activated T-cells (NFAT) activation.
These transcriptional defects are reversed by transfection of P98 cells with a wild-type PLC-gamma1 expression vector.
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| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
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| Subculturing |
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2 X 105 viable cells/mL.
Maintain cell density between 1 X 105 and 2 to 3 X 106 viable cells/mL. |
| Cryopreservation |
culture medium 95%; DMSO, 5% |
| Culture Conditions |
Temperature: 37°C |
| STR Profile |
Amelogenin: X CSF1PO: 11,12 D13S317: 8,12 D16S539: 11 D5S818: 9 D7S820: 8,12 THO1: 6,9.3 TPOX: 8,10 vWA: 18,19 |
| Name of Depositor |
RT Abraham |
| Deposited As |
human |
| References |
Irvin BJ, et al. Pleiotropic contributions of phospholipase C-gamma1 (PLC-gamma1) to T-cell antigen receptor-mediated signaling reconstitution studies of a PLC-gamma1-deficient Jurkat T-cell line. Mol. Cell. Biol. 20: 9149-9161, 2000. PubMed: 11094067
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