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J.RT3-T3.5

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編號:B164869

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產品名稱	J.RT3-T3.5
商品貨號	B164869
Organism	Homo sapiens, human
Tissue	peripheral blood
Cell Type	T lymphocyte
Product Format	frozen
Morphology	lymphoblast
Culture Properties	suspension
Biosafety Level	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease	acute T cell leukemia
Gender	male
Applications	transfection host
Storage Conditions	liquid nitrogen vapor phase
Derivation	The J.RT3-T3.5 cell line is a derivative mutant of the Jurkat leukemia cell line. The Jurkat cell line was established from the peripheral blood of a 14 year old boy by Schneider et al., and was originally designated JM. The line was produced by treatment with ethylmethanesulfonate and negative selection with OKT3 monoclonal antibody. This is a mutant line derived from the E6-1 clone of Jurkat (ATCC TIB 152) that lacks the beta chain of the T cell antigen receptor.
Clinical Data	male The Jurkat cell line was established from the peripheral blood of a 14 year old boy by Schneider et al.
Comments	J.RT3-T3.5 cells lack the beta chain of the T cell antigen receptor. The cells do not express either CD3 or the T cell receptor alpha/beta heterodimer on the surface. The cells may be useful for transfection studies involving the T cell receptor beta chain gene.
Complete Growth Medium	The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
Subculturing	Medium Renewal: 3 times per week Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1 x 10⁵ cells/mL and maintain between 1 x 10⁵ and 1 x 10⁶ cells/mL. Do not allow the cell concentration to exceed 1 x 10⁶ cells/mL.
Cryopreservation	culture medium 95%; DMSO, 5%
Culture Conditions	Temperature: 37.0°C
Name of Depositor	A Weiss
Deposited As	Homo sapiens
References	Ohashi PS, et al. Reconstitution of an active surface T3/T-cell antigen receptor by DNA transfer. Nature 316: 606-609, 1985. PubMed: 4033759 Weiss A, Stobo JD. Requirement for the coexpression of T3 and the T cell antigen receptor on a malignant human T cell line. J. Exp. Med. 160: 1284-1299, 1984. PubMed: 6208306 Schneider U, et al. Characterization of EBV-genome negative "null" and "T" cell lines derived from children with acute lymphoblastic leukemia and leukemic transformed non-Hodgkin lymphoma. Int. J. Cancer 19: 621-626, 1977. PubMed: 68013 Ronald Wange, personal communication

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