| 產(chǎn)品名稱(chēng) |
JES3-19F1.1.1 |
| 商品貨號(hào) |
B164891 |
| Organism |
Rattus norvegicus (B cell); Mus musculus (myeloma), rat (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications |
The antibody blocks the IL-10 mediated inhibition of gamma interferon production by PHA stimulated human peripheral blood leukocytes. |
| Disclosure |
This material is cited in a US or other Patent and may not be used to
infringe the claims. Depending on the wishes of the Depositor, ATCC may be
required to inform the Patent Depositor of the party to which the material was
furnished. This material may not have been produced or characterized by ATCC. |
| Derivation |
Spleen cells were fused with P3X63Ag8.653 myeloma cells. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against human interleukin 10 (IL-10, CSIF) |
| Cellular Products |
immunoglobulin; monoclonal antibody; against human interleukin 10 (IL-10, CSIF) |
| Comments |
Animals were immunized with human IL-10. Spleen cells were fused with P3X63Ag8.653 myeloma cells. The antibody blocks the IL-10 mediated inhibition of gamma interferon production by PHA stimulated human peripheral blood leukocytes. |
| Complete Growth Medium |
Culture Medium: RPMI 1640 medium with 1 mM L-glutamine, 90%; fetal bovine
serum, 10%. |
| Subculturing |
Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Culture Conditions |
Temperature: 37.0°C |
| Isotype |
IgG2a |
| Name of Depositor |
Schering Corp. |
| U.S. Patent Number |
|
