| 產(chǎn)品名稱 |
KC-4G3 [KC-4(IgG3)] |
| 商品貨號 |
B164905 |
| Organism |
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease |
Adenocarcinoma |
| Applications |
The antibody reacts with an antigen present on solid tumors (98 of 104 tested) and cross-reacts with minimal cross reaction with paraffin embedded (14 of 92) or frozen (1 of 33) normal tissues. An antibody with the same specificity but of the IgM class is available (ATCC HB-8710). |
| Disclosure |
This material is cited in a US or other Patent and may not be used to
infringe the claims. Depending on the wishes of the Depositor, ATCC may be
required to inform the Patent Depositor of the party to which the material was
furnished. This material may not have been produced or characterized by ATCC. |
| Derivation |
Spleen cells were fused with Sp2/0-Ag14 myeloma cells. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against human carcinoma cells |
| Cellular Products |
immunoglobulin; monoclonal antibody; against human carcinoma cells |
| Comments |
Animals were immunized with prostatic adenocarcinoma cells. Spleen cells were fused with Sp2/0-Ag14 myeloma cells. The antibody reacts with an antigen present on solid tumors (98 of 104 tested) and cross-reacts with minimal cross reaction with paraffin embedded (14 of 92) or frozen (1 of 33) normal tissues. The antigen is found in the cytoplasm as a 490000 dalton molecule, and in the membrane and cytoplasm as a 438000 dalton molecule. An antibody with the same specificity but of the IgM class is available (ATCC HB-8710). |
| Complete Growth Medium |
Culture Medium: Modified Dulbecco's medium (see below)with 0.1 mM hypoxanthine
and 0.015 mM thymidine, 85%; fetal bovine serum, 15%. |
| Subculturing |
Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Cryopreservation |
fetal bovine serum, 90%; DMSO, 10% |
| Isotype |
IgG3 |
| Name of Depositor |
Coulter Corporation |
| Deposited As |
mouse (B cell); mouse (myeloma) |
| U.S. Patent Number |
|
| References |
Kortright KH, Hofheinz DE. Monoclonal antibody to a human carcinoma tumor associated antigen. US Patent 4,708,930 dated Nov 24 1987
|
