| 產品名稱 |
KHOS/NP (R-970-5) |
| 商品貨號 |
B164911 |
| Organism |
Homo sapiens, human |
| Tissue |
bone |
| Product Format |
frozen |
| Culture Properties |
adherent |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease |
osteosarcoma |
| Age |
13 years |
| Gender |
female |
| Ethnicity |
Caucasian |
| Applications |
The cells are useful producing MSV pseudotypes with various ecotropic and xenotropic murine leukemia viruses. |
| Storage Conditions |
liquid nitrogen vapor phase |
| Derivation |
This cell line was derived from HOS (ATCC CRL-1543) by transformation using Kirsten murine sarcoma virus (Ki-MSV). |
| Clinical Data |
female Caucasian 13 years |
| Tumorigenic |
Yes |
| Effects |
Yes, Tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 10(7) cells. |
| Comments |
The cells exhibit a high saturation density, a high plating efficiency in soft agar and produce tumors in nude mice.
Cells carry the Ki-MSV genome but do not produce infectious virus particles or viral antigens. |
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing |
Remove medium, add fresh 0.25% trypsin solution for 2 to 3 minutes, remove trypsin and let the culture sit at room temperature until the cells detach. Add fresh medium, aspirate and dispense into new flasks.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: 2 to 3 times per week |
| STR Profile |
Amelogenin: X CSF1PO: 12 D13S317: 12 D16S539: 10,13 D5S818: 13 D7S820: 11,12 THO1: 6 TPOX: 8,11 vWA: 18 |
| Name of Depositor |
JS Rhim |
| Deposited As |
Homo sapiens |
| References |
Rhim JS, et al. Non-producer human cells induced by murine sarcoma virus. Int. J. Cancer 15: 23-29, 1975. PubMed: 165148
Rhim JS, et al. Characterization of non-producer human cells induced by Kirsten sarcoma virus. Int. J. Cancer 16: 840-849, 1975. PubMed: 171229
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