| 產品名稱 |
KL304 |
| 商品貨號 |
B164916 |
| Organism |
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications |
Animals were immunized with synthetic peptide corresponding to residues 146 to 157 of the mouse As/beta protein (domain beta 1). The antibody reacts with denatured beta chains of the a, f, k, j, s and bw haplotypes. |
| Storage Conditions |
liquid nitrogen vapor phase |
| Derivation |
Spleen cells were fused with Sp2/0-Ag14 myeloma cells. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against the beta chain of mouse Class II antigens |
| Cellular Products |
immunoglobulin; monoclonal antibody; against the beta chain of mouse Class II antigens |
| Comments |
Animals were immunized with synthetic peptide corresponding to residues 146 to 157 of the mouse As/beta protein (domain beta 1). Spleen cells were fused with Sp2/0-Ag14 myeloma cells. The antibody reacts with denatured beta chains of the a, f, k, j, s and bw haplotypes. It does not detect the intact H-2b Class II molecule, and does not detect the beta chain on the cell surface. It does not react with molecules of the b, d, p or q haplotypes. The reactive determinant most likely involves residues 153 through 155. |
| Complete Growth Medium |
Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose and supplemented with 1% nonessential amino acids, 10 mM HEPES, 0.01 mM 2-mercaptoethanol and 10% fetal bovine serum
|
| Subculturing |
Protocol: Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 X 10(5) viable cells/ml. Do not allow the cell concentration to exceed 1 X 10(6) cells/ml.
Subcultivation Ratio: Maintain cultures at a cell concentration between 1 X 10(5) and 1 X 10(6) cells/ml. Medium Renewal: Every 2 to 3 days |
| Cryopreservation |
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions |
Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0°C |
| Isotype |
IgG2b |
| Name of Depositor |
JA Frelinger |
| Deposited As |
mouse (B cell); mouse (myeloma) |
| References |
LaPan KE, et al. Production and characterization of two new mouse monoclonal antibodies reactive with denatured mouse class II beta chains. Hybridoma 11: 217-223, 1992. PubMed: 1376718
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