| 產品名稱 |
L.N. 4159 |
| 商品貨號 |
B164932 |
| Organism |
Mus musculus, mouse |
| Tissue |
liver |
| Product Format |
frozen |
| Culture Properties |
adherent |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disclosure |
This material is cited in a US or other Patent and may not be used to
infringe the claims. Depending on the wishes of the Depositor, ATCC may be
required to inform the Patent Depositor of the party to which the material was
furnished. This material may not have been produced or characterized by ATCC. |
| Genes Expressed |
temperature sensitive T antigen |
| Cellular Products |
temperature sensitive T antigen |
| Comments |
The cells contain the HB101 pLux F70 plasmid. The cells should be grown at 33C. |
| Complete Growth Medium |
Dulbecco's modified Eagle's medium with 200 nM dexamethasone, 96%; fetal bovine serum, 4%
|
| Subculturing |
Medium Renewal: Every 2 to 3 days Remove medium, rinse monolayer with fresh 0.25% trypsin solution, remove trypsin and let the culture sit at room temperature for 5 to 10 minutes. Add fresh medium, aspirate and dispense into new flasks. |
| Name of Depositor |
Pfizer, Inc. |
| Deposited As |
Mus musculus |
