| 產品名稱 |
L11/135 |
| 商品貨號 |
B164933 |
| Organism |
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications |
The antibody reacts with an antigen present on all rabbit T cells. Tested and found negative for ectromelia virus (mousepox). |
| Derivation |
Spleen cells were fused with P3X63Ag8-U1 myeloma cells. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against rabbit T lymphocytes (T cells) |
| Cellular Products |
immunoglobulin; monoclonal antibody; against rabbit T lymphocytes (T cells) |
| Comments |
Animals were immunized with cell surface glycoproteins of the rabbit T cell line, RL-5. Spleen cells were fused with P3X63Ag8-U1 myeloma cells. The antibody reacts with an antigen present on all rabbit T cells. Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium |
The base medium for this cell line is ATCC Hybri-Care Medium, Catalog No. 46-X. Hybri-Care Medium is supplied as a powder and should be reconstituted in 1 L cell culture grade water. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10% 1.5 g/L sodium bicarbonate for use with 5% CO2 in air atmosphere.
|
| Subculturing |
Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1 x 10 exp5 cells/ml and maintain between 1 x 10 exp5 and 1 x 10 exp6 cells/ml. |
| Isotype |
IgG1 |
| Name of Depositor |
S Jackson |
| Deposited As |
mouse (B cell); mouse (myeloma) |
| References |
Jackson S, et al. Differentiation antigens identify subpopulations of rabbit T and B lymphocytes. J. Exp. Med. 157: 34-46, 1983. PubMed: 6600269
|
