| 產(chǎn)品名稱 |
L243 |
| 商品貨號(hào) |
B164941 |
| Organism |
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type |
Hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
Lymphoblast-like |
| Culture Properties |
Suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications |
The antibody reacts with a non-polymorphic Ia like antigen on human cells. |
| Derivation |
Animals were immunized with RPMI 8866 cells. Spleen cells were fused with NS-1 myeloma cells. |
| Cellular Products |
immunoglobulin; monoclonal antibody; against human Ia; cytotoxic |
| Comments |
An antibody with similar specificity but not cytotoxic is available (ATCC HB-171, L203).
Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium |
The base medium for this cell line is ATCC Hybri-Care Medium, Catalog No. 46-X. Hybri-Care Medium is supplied as a powder and should be reconstituted in 1 L cell culture grade water. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10% 1.5 g/L sodium bicarbonate for use with 5% CO2 in air atmosphere.
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| Subculturing |
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 105 cells/mL and maintain between 1 X 105 and 1 X 106 cells/mL.
Medium Renewal: Every 2 to 3 days |
| Isotype |
IgG2a |
| Antigenic Determinant |
I-A, human |
| Name of Depositor |
LA Lampson |
| Deposited As |
mouse (B cell); mouse (myeloma) |
| References |
Lampson LA, Levy R. Two populations of Ia-like molecules on a human B cell line. J. Immunol. 125: 293-299, 1980. PubMed: 6966655
Nepom BS, et al. Critical contribution of beta chain residue 57 in peptide binding ability of both HLA-DR and -DQ molecules. Proc. Natl. Acad. Sci. USA 93: 7202-7206, 1996. PubMed: 8692969
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