| 產(chǎn)品名稱 |
LA-4 |
| 商品貨號(hào) |
B164954 |
| Organism |
Mus musculus, mouse |
| Tissue |
lung |
| Cell Type |
urethane induced |
| Product Format |
frozen |
| Morphology |
epithelial |
| Culture Properties |
adherent |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease |
adenoma |
| Age |
28 weeks |
| Strain |
A/He |
| Storage Conditions |
liquid nitrogen vapor phase |
| Karyotype |
Unstable karyotype. Range = 38 to 256. Metacentric chromosomes are noted in about 12% of the cells. Note: Cytogenetic information is based on initial seed stock at ATCC. Cytogenetic instability has been reported in the literature for some cell lines. |
| Comments |
Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium |
Ham's F12K medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 85%; fetal bovine serum, 15%
|
| Subculturing |
Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
- Remove and discard culture medium.
-
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
-
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
-
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
-
Add appropriate aliquots of the cell suspension to new culture vessels.
- Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended
Medium Renewal: Twice per week |
| Cryopreservation |
Freeze medium: Complete culture medium 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions |
Temperature: 37.0°C |
| Name of Depositor |
GD Stoner |
| Deposited As |
Mus musculus |
| References |
Stoner GD, et al. Clonal isolation of epithelial cells from mouse lung adenoma. Cancer Res. 35: 2177-2185, 1975. PubMed: 167947
Stoner GD, et al. Epithelial cells cultured from mouse lung adenoma: biosynthesis of lecithin and comparison of phospholipids with the lung. Exp. Mol. Pathol. 29: 102-114, 1978. PubMed: 581072
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