| 產品名稱 |
LK-4 |
| 商品貨號 |
B164976 |
| Organism |
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications |
The hybridoma cell line LK-4 secretes a mouse monoclonal antibody (IgG1k) that recognizes human platelet glycoprotein GPIIIa. The antibody can differentiate platelet PLA1/PLA1, PLA1/PLA2 and PLA2/PLA2 genotypes on soluble platelet extracts; it can recognize but not differentiate intact platelets. The antibody is useful in immunoblot and ELISA assays. The line was produced by fusing P3U1 mouse myeloma cells with spleen cells from a BALB/c female mouse differentially immunized with human PLA2 platelets and cyclophosphamide, followed by PLA1 platelets. |
| Derivation |
The line was produced by fusing P3U1 mouse myeloma cells with spleen cells from a BALB/c female mouse differentially immunized with human PLA2 platelets and cyclophosphamide, followed by PLA1 platelets. |
| Clinical Data |
The line was produced by fusing P3U1 mouse myeloma cells with spleen cells from a BALB/c female mouse differentially immunized with human PLA2 platelets and cyclophosphamide, followed by PLA1 platelets. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against human platelet glycoprotein GPIIIa. |
| Cellular Products |
immunoglobulin; monoclonal antibody; against human platelet glycoprotein GPIIIa. |
| Comments |
The hybridoma cell line LK-4 secretes a mouse monoclonal antibody (IgG1k) that recognizes human platelet glycoprotein GPIIIa. The antibody can differentiate platelet PLA1/PLA1, PLA1/PLA2 and PLA2/PLA2 genotypes on soluble platelet extracts; it can recognize but not differentiate intact platelets. The antibody inhibits binding of human anti- PLA1 antibody to human platelets. The antibody is useful in immunoblot and ELISA assays. The line was produced by fusing P3U1 mouse myeloma cells with spleen cells from a BALB/c female mouse differentially immunized with human PLA2 platelets and cyclophosphamide, followed by PLA1 platelets. |
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
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| Subculturing |
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density) Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2 X 10 exp5 viable cells/ml. Maintain cell density between 1 X 10 exp5 and 1 X 10 exp6 viable cells/ml. |
| Cryopreservation |
culture medium 95%; DMSO, 5% |
| Culture Conditions |
Temperature: 37.0°C |
| Isotype |
IgG1; kappa light chain |
| Name of Depositor |
S Karpatkin |
| Deposited As |
mouse (B cell); mouse (myeloma) |
| References |
Liu LX, et al. A monoclonal antibody (LK-4) which differentiates PLA1 from PLA2 platelet extracts but not intact platelets. Thromb. Res. 66: 309-320, 1992. PubMed: 1384161
Liu LX, et al. Development of a monoclonal antibody capable of differentiating platelet PLA1/PLA1, PLA1/PLA2 and PLA2/PLA2 genotypes. Br. J. Haematol. 81: 113-117, 1992. PubMed: 1520609
Liu LX, et al. Heterogenous inhibition of platelet aggregation by monoclonal antibodies binding to multiple sites on GPIIIa. Br. J. Haematol. 91: 976-982, 1995. PubMed: 8547151
Valetin N, et al. Involvement of the cysteine-rich domain of glycoprotein IIIa in the expression of the human platelet alloantigen, PlA1: evidence for heterogeneity in the humoral response. Blood 85: 3028-3033, 1995. PubMed: 7756638
Liu LX, et al. Inhibition of binding of anti-PLA1 antibodies to platelets with monoclonal antibody LK-4. Evidence for multiple PLA1 receptor sites on platelet GPIIIa. Blood 88: 3601-3607, 1996. PubMed: 8896429
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