| Comments |
Animals were immunized with stromal cells from mouse bone marrow. Spleen cells were fused with Sp2/0 myeloma cells. The antibody blocks adhesion of lymphocyte precursors to bone marrow stromal cells. The antibody recognizes an antigen believed to be the mouse analog of human VCAM-1 that is expressed on stromal cells in bone marrow and on inflamed endothelial cells (see also M/K-1.9, ATCC CRL-1910). |
| References |
Miyake K, et al. A VCAM-like adhesion molecule on murine bone marrow stromal cells mediates binding of lymphocyte precursors in culture. J. Cell Biol. 114: 557-565, 1991. PubMed: 1713592
Miyake K, et al. Evidence for a role of the integrin VLA-4 in lympho-hemopoiesis. J. Exp. Med. 173: 599-607, 1991. PubMed: 1997648
Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.
Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.
Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.
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