| 產品名稱 |
M17/5.2 |
| 商品貨號 |
B165043 |
| Organism |
Rattus norvegicus (B cell); Mus musculus (myeloma), rat (B cell); mouse (myeloma) |
| Tissue |
spleen |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications |
The antibody blocks CTL mediated cytotoxicity, and can be used to precipitate LFA-1 from lysates of CTLs. |
| Storage Conditions |
liquid nitrogen vapor phase |
| Derivation |
Spleen cells were fused with NS-1 myeloma cells. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against mouse lymphocyte function antigen 1 (LFA-1, CD11a) |
| Cellular Products |
immunoglobulin; monoclonal antibody; against mouse lymphocyte function antigen 1 (LFA-1, CD11a) |
| Comments |
Animals were immunized with mouse cytotoxic T lymphocytes (CTL). Spleen cells were fused with NS-1 myeloma cells. The antibody blocks CTL mediated cytotoxicity, and can be used to precipitate LFA-1 from lysates of CTLs. |
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing |
Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 X 105 viable cells/mL.
Interval: Maintain between 1 X 105 and 1 X 106 cells/mL.
Medium Renewal: Every 2 to 3 days |
| Cryopreservation |
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions |
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C |
| Isotype |
rat IgG2b kappa |
| Name of Depositor |
TA Springer |
| Deposited As |
rat (B cell); mouse (myeloma) |
| References |
Sanchez-Madrid F, et al. Antigens involved in mouse cytolytic T-lymphocyte (CTL)-mediated killing: functional screening and topographic relationship. Cell. Immunol. 73: 1-11, 1982. PubMed: 6983917
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