| 產品名稱 |
mAb BB7 |
| 商品貨號 |
B165070 |
| Organism |
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications |
The antibody is specific for polypyrimidine tract binding protein (PTB). The antibody may be used in immunodepletion experiments. |
| Derivation |
Spleen cells were fused with Sp2/0 mouse myeloma cells. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against polypyrimidine tract binding protein (PTB) |
| Cellular Products |
immunoglobulin; monoclonal antibody; against polypyrimidine tract binding protein (PTB) |
| Comments |
Animals were immunized with bacterially expressed full length GST-PTB recombinant protein. Spleen cells were fused with Sp2/0 mouse myeloma cells. The antibody is specific for polypyrimidine tract binding protein (PTB). Polypyrimidine tract binding protein (PTB) is a represssor of pre-mRNA splicing. The antibody may be used in immunodepletion experiments. |
| Complete Growth Medium |
Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose and supplemented with 10 mM HEPES, 0.1 mM nonessential amino acids and 0.05 mM 2-mercaptoethanol, 80%; fetal bovine serum, 20%
|
| Subculturing |
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density) Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2 X 10 exp5 viable cells/ml. Maintain cell density between 1 X 10 exp5 and 1 X 10 exp6 viable cells/ml. |
| Cryopreservation |
culture medium 95%; DMSO, 5% |
| Culture Conditions |
Temperature: 37.0°C |
| Isotype |
IgG2b; kappa light chain |
| Name of Depositor |
DL Black |
| Deposited As |
mouse (B cell); mouse (myeloma) |
