| 產(chǎn)品名稱 | Malme-3M |
|---|---|
| 商品貨號 | B165075 |
| Organism | Homo sapiens, human |
| Tissue | malignant melanoma; Derived from metastatic site: lung |
| Cell Type | fibroblast |
| Product Format | frozen |
| Morphology | mixed |
| Culture Properties | mixed adherent-suspension |
| Biosafety Level | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease | Malignant melanoma |
| Age | 43 years |
| Gender | male |
| Ethnicity | Caucasian |
| Applications | This melanoma cell line was isolated from the same patient as Malme-3 (ATCC HTB-102), a normal skin fibroblast. The two cell lines provide tumor and normal counterparts for comparative in vitro melanoma studies. |
| Storage Conditions | liquid nitrogen vapor phase |
| Karyotype | modal number = 88; range = 76 to 93 This is a hypotetraploid human cell line with the modal chromosome number of 88 occurring in 17% of cells. Cells having 82 to 87 chromosome counts were also abundant. Six marker chromosomes were common to most cells, including i(1q), i(6p), i(7q), t(4qter--4pter::?::4q11--4qter) and two others. Except for the paired i(7q), all were single. Two copies of normal X and Y chromosomes were detected in most cells. Normal chromosomes N3 and N17 had five copies in most cells. |
| Images |  |
| Derivation | This is one of an extensive series of human tumor lines isolated and characterized by J. Fogh. This melanoma cell line was isolated from the same patient as Malme-3 (ATCC HTB-102), a normal skin fibroblast. - |
| Clinical Data | Caucasian male This melanoma cell line was isolated from the same patient as Malme-3 (ATCC HTB-102), a normal skin fibroblast. 43 years |
| Antigen Expression | Antigen expression: HLA A2, Aw30, B13, B40(+/-), DRw7 |
| Tumorigenic | Yes |
| Effects | Yes, in nude mice; forms pigmented malignant melanoma |
| Complete Growth Medium | The base medium for this cell line is ATCC-formulated Iscove's Modified Dulbecco's Medium, Catalog No. 30-2005. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%.
|
| Subculturing |
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: Two to three times weekly |
| Cryopreservation | Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions | Temperature: 37°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5% |
| STR Profile | Amelogenin: X,Y CSF1PO: 12 D13S317: 8,13 D16S539: 9,12 D5S818: 11 D7S820: 9,12 THO1: 8 TPOX: 8,9 vWA: 15,16 |
| Isoenzymes | AK-1, 1 ES-D, 1 G6PD, B GLO-I, 1-2 Me-2, 1 PGM1, 1 PGM3, 1 |
| Name of Depositor | J Fogh |
| Deposited As | Homo sapiens |
| References | Fogh J. Human tumor cells in vitro. New York: Plenum Press; 1975. Fogh J, et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214, 1977. PubMed: 833871 Goodfellow M, et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J. Natl. Cancer Inst. 59: 221-226, 1977. PubMed: 77210034 Pollack MS, et al. HLA-A, B, C and DR alloantigen expression on forty-six cultured human tumor cell lines. J. Natl. Cancer Inst. 66: 1003-1012, 1981. PubMed: 7017212 |
| 梅經(jīng)理 | 17280875617 | 1438578920 |
| 胡經(jīng)理 | 13345964880 | 2438244627 |
| 周經(jīng)理 | 17757487661 | 1296385441 |
| 于經(jīng)理 | 18067160830 | 2088210172 |
| 沈經(jīng)理 | 19548299266 | 2662369050 |
| 李經(jīng)理 | 13626845108 | 972239479 |
