| Applications |
The antibody is specific for E. coli O157 O-antigen and for the Salmonella serogroup N O-antigen (which is identical to the E. coli O157 antigen). High titer ascites can be produced but these cells become lethal to mice within approximately 5 days post-intraperitioneal injection. The antibody has been used in ELISA, Western Blot, lateral flow and chemiluminescense assays. |
| Comments |
Animals were immunized with whole bacterium antigen from E. coli O157:H7 (ATCC 43895). Spleen cells were fused with P3X63Ag8.653 mouse myeloma cells. The antibody is specific for E. coli O157 O-antigen and for the Salmonella serogroup N O-antigen (which is identical to the E. coli O157 antigen). Both ascites and culture media react with extremely high affinity. High titer ascites can be produced but these cells become lethal to mice within approximately 5 days post-intraperitioneal injection. The antibody has been used in ELISA, Western Blot, lateral flow and chemiluminescense assays. |
| References |
Westerman RB, et al. Production and characterization of monoclonal antibodies specific for the lipopolysaccharide of Escherichia coli O157. J. Clin. Microbiol. 35: 679-684, 1997. PubMed: 9041412
Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.
Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.
Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.
Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online.
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