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MC/CAR

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編號:B165097

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產品名稱	MC/CAR
商品貨號	B165097
Organism	Homo sapiens, human
Tissue	peripheral blood
Cell Type	B lymphocyte
Product Format	frozen
Morphology	lymphoblast
Culture Properties	suspension
Biosafety Level	2 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease	plasmacytoma; myeloma
Age	81 years
Gender	male
Ethnicity	Caucasian
Applications	Can be used as a human fusion line.
Storage Conditions	liquid nitrogen vapor phase
Disclosure	This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Karyotype	normal male diploid
Derivation	Although derived from cells taken from a patient with a plasmacytoma, this line has been shown to be an EBV-transformed B lymphoblastoid cell line.
Clinical Data	male Caucasian 81 years
Antigen Expression	HLA A1, A33/31, B7, B18, DRw1, DRw2; CD11a +; CD19 +; CD20 + CD28 -; CD38 +
Receptor Expression	complement (C3b)
Genes Expressed	immunoglobulin
Cellular Products	immunoglobulin
Tumorigenic	Yes
Effects	Yes, in nude mice inoculated subcutaneously with 10⁷ cells (Tumors developed within 21 days at 100% frequency (5/5).)
Comments	The cells produce, but do not secrete, immunoglobulin. No surface immunoglobulin. 8-azaguanine mutants may be cloned out or mutagenized for HAT sensitive cultures.
Complete Growth Medium	Iscove's modified Dulbecco's medium, 80%; fetal bovine serum, 20%
Subculturing	Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 5 x 10⁵ cells/mL and maintain between 5 x 10⁵and 1 x 10⁶cells/mL. Medium Renewal: Two to three times weekly.
Culture Conditions	Temperature: 37°C
STR Profile	Amelogenin: X,Y CSF1PO: 11,12 D13S317: 11,13 D16S539: 11,12 D5S818: 11,14 D7S820: 9,13 THO1: 6,9 TPOX: 8 vWA: 17,18
Isoenzymes	ADA, 1 AK-1, 1 ES-D, 1 G6PD, B GLO-I, 1 PGM1, 1 PGM3, 1
Isotype	IgG1 (cytoplasmic); kappa light chain
Name of Depositor	Research Corporation
U.S. Patent Number	4,434,230
References	Ritts RE Jr.. Human nonsecretory plasmacytoid cell line. US Patent 4,434,230 dated Feb 28 1984 Pellat-Deceunynk C, et al. Human myeloma cell lines as a tool for studying the biology of multiple myeloma: a reappraisal 18 years after. Blood 86: 4001-4002, 1995. PubMed: 7579375 Drexler H, et al. False human hematopoietic cell lines: cross-contaminations and misinterpretations. Leukemia 13: 1601-1607, 1999. PubMed: 10516762 Ritts RE J, et al. Establishment and characterization of a human non-secretory plasmacytoid cell line and its hybridization with human B cells. Int. J. Cancer 31: 133-141, 1983. PubMed: 6600719 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

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