| 產品名稱 |
McA-RH8994 |
| 商品貨號 |
B165105 |
| Organism |
Rattus norvegicus, rat |
| Tissue |
liver |
| Product Format |
frozen |
| Morphology |
epithelial |
| Culture Properties |
adherent |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease |
hepatoma; Morris hepatoma 8994 |
| Gender |
male |
| Storage Conditions |
liquid nitrogen vapor phase |
| Clinical Data |
male |
| Receptor Expression |
glucocorticoid |
| Genes Expressed |
alpha-fetoprotein (AFP) |
| Cellular Products |
alpha-fetoprotein (AFP); alpha fetoprotein |
| Comments |
Addition of glucocorticoids induces alpha fetoprotein synthesis and slows cell proliferation.
AFP is also synthesized when cell growth slows as the cells end the log phase of growth. |
| Complete Growth Medium |
Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose, 75%; horse serum, 20%; fetal bovine serum, 5%.
|
| Subculturing |
Remove medium, add fresh 0.25% trypsin solution, remove trypsin immediately and let the culture sit at room temperature for 5 to 10 minutes. Add fresh medium, aspirate and dispense into new flasks. Subculture once per week.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: Every 2 or 3 days |
| Name of Depositor |
JE Becker |
| Deposited As |
Rattus sp. |
| References |
Morris H, Meranze D. Induction and Some Characteristics of “Minimal Deviation” and Other Transplantable Rat Hepatomas. Recent Results Cancer Res. 44: 103-114, 1974.
Becker JE, et al. Two new rat hepatoma cell lines for studying the unbalanced blocked ontogeny hypothesisIn: Becker JE, et al. Onco-developmental gene expression. New YorkAcademic Press, pp. 259-270, 1976
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