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MECA-79

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編號:B165140

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產品名稱	MECA-79
商品貨號	B165140
Organism	Rattus norvegicus (B cell); Mus musculus (myeloma), rat (B cell); mouse (myeloma)
Cell Type	hybridoma: B lymphocyte
Product Format	frozen
Morphology	lymphoblast
Culture Properties	suspension
Biosafety Level	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Applications	The antibody specifically blocks lymphocyte adhesion to mouse peripheral lymph node endothelia and does not block lymphocyte binding to mouse Peyer's patches. It binds to a sulfate and fucose dependent epitope associated with L-selectin binding ligands of high endothelial venules (HEV).
Disclosure	This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Derivation	Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
Genes Expressed	immunoglobulin; monoclonal antibody; against mouse peripheral lymph node endothelial cells (peripheral node addressin)
Cellular Products	immunoglobulin; monoclonal antibody; against mouse peripheral lymph node endothelial cells (peripheral node addressin)
Comments	Animals were immunized with mouse lymph node stroma. Spleen cells were fused with Sp2/0-Ag14 myeloma cells. The antibody specifically blocks lymphocyte adhesion to mouse peripheral lymph node endothelia and does not block lymphocyte binding to mouse Peyer's patches. It binds to a sulfate and fucose dependent epitope associated with L-selectin binding ligands of high endothelial venules (HEV). It inhibits L-selectin dependent lymphocyte homing to lymph nodes and blocks lymphocyte binding to HEV in vitro. This line grows best when cultured with a feeder layer of mouse (BALB/c) peritoneal exudate cells.
Complete Growth Medium	RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate, 90%; fetal bovine serum, 10%; supplemented with 0.05 mM 2-mercaptoethanol. Note: A feeder layer of mouse peritoneal exudate or irradiated murine fibroblasts (e.g. irradiated STO cells, ATCC catalog # X-56) is required for recovery and growth of the MECA-79.
Subculturing	Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10⁵ cells/mL and maintain between 1 X 10⁵ and 1 X 10⁶ cells/mL.
Culture Conditions	Temperature: 37.0°C
Isotype	IgM
Name of Depositor	Stanford University Board of Trustees
Deposited As	rat (B cell); mouse (myeloma)
U.S. Patent Number	5,403,919
References	Butcher EC. Method to control leukocyte extravasation. US Patent 5,403,919 dated Apr 4 1995 Berg EL, et al. The human peripheral lymph node vascular addressin is a ligand for LECAM-1, the peripheral lymph node homing receptor. J. Cell Biol. 114: 343-349, 1991. PubMed: 1712790 Berg EL, et al. L-selectin-mediated lymphocyte rolling on MAdCAM-1. Nature 366: 695-698, 1993. PubMed: 7505053 Hemmerich S, et al. Sulfation-dependent recognition of high endothelial venules (HEV)- ligands by L-selectin and MECA 79, and adhesion-blocking monoclonal antibody. J. Exp. Med. 180: 2219-2226, 1994. PubMed: 7525849 Michie SA, et al. The human peripheral lymph node vascular addressin. An inducible endothelial antigen involved in lymphocyte homing. Am. J. Pathol. 143: 1688-1698, 1993. PubMed: 8256856 . . Cell. Biol. 107: 1853-1862, 1988.

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