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編號:B165176

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產品名稱	MMQ
商品貨號	B165176
Organism	Rattus norvegicus, rat
Tissue	pituitary
Product Format	frozen
Morphology	epithelial
Culture Properties	suspension
Biosafety Level	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease	tumor
Age	5 days
Applications	The cells secrete prolactin and express functional dopamine receptors [U.S. Patent 5,510,260].
Storage Conditions	liquid nitrogen vapor phase
Disclosure	This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Receptor Expression	dopamine
Genes Expressed	prolactin
Cellular Products	prolactin
Tumorigenic	No
Effects	No, in immunosuppressed mice Yes, in semisolid medium
Comments	The cells secrete prolactin and express functional dopamine receptors [U.S. Patent 5,510,260].
Complete Growth Medium	The base medium for this cell line is ATCC-formulated F-12K Medium, Catalog No. 30-2004. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 2.5% horse serum to a final concentration of 15%
Subculturing	Cultures can be maintained by addition or replacement of medium. Establish new cultures at 2 x 10⁵ viable cells/mL and maintain between 1 x 10⁵ and 1 x 10⁶ cells/mL. Medium Renewal: Every 2 to 3 days Note: The line grows as clumps of cells in suspension.
Cryopreservation	Complete culture medium described above supplemented with 5% (v/v) DMSO. Cell culture tested DMSO is available as ATCC^® Catalog No. 4-X.
Culture Conditions	Temperature: 37°C Atmosphere: Air, 95%; carbon dioxide (CO₂), 5%
Population Doubling Time	24 to 48 hrs
Name of Depositor	University of Virginia Patent Foundation
U.S. Patent Number	5,510,260
References	MacLeod RM, et al. Prolactin secreting cell line and method of obtaining the same. US Patent 5,510,260 dated Apr 23 1996 The MMQ cell line was established from the 7315a rat pituitary tumor. Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

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