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The cells can be propagated in spinner cultures or in static cultures.

The amounts of stored and released somatostatin can be increased 2 to 3 fold by treatment for 4 days with 1 micromolar 5-bromo-2'-deoxyuridine.

Medium Renewal: Every 2 to 3 days

Complete growth medium described above supplemented with 5% (v/v) DMSO.  Cell culture tested DMSO is available as ATCC Catalog No. 4-X.

Tischler AS, et al. Establishment of a continuous somatostatin-producing line of medullary thyroid carcinoma cells from BALB/c mice. J. Endocrinol. 110: 309-313, 1986. PubMed: 2875121

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online.