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The consistency of the line's characteristics has made it a useful tool in a wide variety of biochemical, immunological, virological and cell biological studies such as being used as a target cell for EBV superinfection studies. It also has proven useful in chemical induction studies of the latent EBV genome and in the propagation of various (retroviruses) oncornaviruses.

Each cell contains an average of 60 EBV genome copies.

The cells are EBNA positive (EBNA+) and surface immunoglobulin negative (sIg-).

Durr FE, et al. Studies on the infectivity and cytopathology of Epstein-Barr virus in human lymphoblastoid cells. Int. J. Cancer 6: 436-449, 1970. PubMed: 4321017

Mayyasi SA, et al. The coating reaction of the herpes-type virus isolated from malignant tissues with an antibody present in sera. Cancer Res. 27: 2020-2024, 1967. PubMed: 6073499

The NC-37 cell line was reportedly initiated by W. Korol from peripheral blood from a donor whose serum was positive for EBV antibodies as determined by immunofluorescence and virus coating test.

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.