B lymphoblast; Epstein-Barr virus (EBV) transforme
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
2 [Cells contain Epstein-Barr Virus]
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Age
49 years
Gender
female
Ethnicity
Caucasian, White
Clinical Data
female
Caucasian, White
49 years
Complete Growth Medium
The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
Subculturing
Medium Renewal: Add fresh medium every 3 to 4 days (depending on cell density)
Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 X 10 exp5 viable cells/ml.
Maintain cell density between 1 X 10 exp6 and 3 X 10 exp6 viable cells/ml.
Cryopreservation
Culture medium, 95%; DMSO, 5%
Culture Conditions
Temperature: 37.0°C
STR Profile
Amelogenin: X
CSF1PO: 10
D13S317: 11
D16S539: 11,13
D5S818: 11,13
D7S820: 10,12
THO1: 7,9.3
TPOX: 8,11
vWA: 17,19
Name of Depositor
AF Gazdar, JD Minna
Deposited As
Homo sapiens
References
NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996.
