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NCI-BL2052 [BL2052]

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編號:B165239

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產(chǎn)品名稱	NCI-BL2052 [BL2052]
商品貨號	B165239
Organism	Homo sapiens, human
Tissue	peripheral blood
Cell Type	B lymphoblast; Epstein-Barr virus (EBV) transformed
Product Format	frozen
Morphology	lymphoblast
Culture Properties	suspension
Biosafety Level	2 [Cells contain Epstein-Barr Virus] Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease	normal
Age	65 years
Gender	male
Ethnicity	Caucasian, White
Storage Conditions	liquid nitrogen vapor phase
Clinical Data	65 years Caucasian male
Comments	A cell line derived from pleural effusion cells from this patient with mesothelioma is available as ATCC CRL-5915. ATCC confirmed this cell line tested positive for the presence of EBV viral DNA sequences via PCR.
Complete Growth Medium	The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
Subculturing	Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 x 10⁵viable cells/mL. Maintain cell density between 1 x 10⁶ and 3 x 10⁶ viable cells/mL. Medium Renewal: Add fresh medium every 3 to 4 days (depending on cell density)
Cryopreservation	Culture medium, 95%; DMSO, 5%. Cell culture tested DMSO is available as ATCC Catalog No. 4-X.
Culture Conditions	Temperature: 37°C
Name of Depositor	AF Gazdar, JD Minna
Deposited As	Homo sapiens
References	NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996. Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

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