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NCI-BL2122 [BL2122]

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編號:B165243

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產品名稱	NCI-BL2122 [BL2122]
商品貨號	B165243
Organism	Homo sapiens, human
Tissue	peripheral blood
Cell Type	B lymphoblast; Epstein-Barr virus (EBV) transformed
Product Format	frozen
Morphology	lymphoblast
Culture Properties	suspension
Biosafety Level	2 [Cells contain Epstein-Barr Virus (Herpesvirus)] Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease	Adenocarcinoma
Age	46 years
Gender	female
Ethnicity	Caucasian
Storage Conditions	liquid nitrogen vapor phase
Derivation	NCI-H2122 was derived from pleural effusion from a 46 year old White female with adenocarcinoma of the lung. NCI-BL2122 BL is a B lymphoblastoid cell line initiated by Epstein-Barr virus (EBV) transformation of peripheral blood lymphocytes obtained from the same patient as NCI-H2122.
Clinical Data	46 years Caucasian female
Comments	The tumor cell line from this patient is available as ATCC CRL-5985.
Complete Growth Medium	The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
Subculturing	Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 5 to 7 x 10⁵ viable cells/mL. A maximum of 1 to 2 x 10⁶cells/mL is obtainable. Cultures grow as floating aggregates of large cell clusters. Add medium as cell density increases. Double volume approximately every 6 to 7 days. Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Cryopreservation	Culture medium, 95%; DMSO, 5%. Cell culture tested DMSO is available as ATCC Catalog No. 4-X.
Culture Conditions	Temperature: 37°C
STR Profile	Amelogenin: X CSF1PO: 10 D13S317: 9,10 D16S539: 9,12 D5S818: 11,12 D7S820: 8,10 THO1: 7,9.3 TPOX: 9 vWA: 17
Name of Depositor	AF Gazdar, JD Minna
Deposited As	Homo sapiens
References	NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996. Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

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